XB-CLONE-3539167
Clone Name: IMAGE:7719634
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Gene:
eif3l
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX982968 [+]
tttttaatttctttacattggacttattattgcatttgcaaaaccttaaatgattgccatgtggaactatttaggttgaggaaaatgcctaaatatgttt
acagtatttgcttcttttcttgagcctgataatctttgtataccaatattaatgtctgaaaacagaatgctcctgtagaaaatatgtggatttattaggc
aaaacatttcaactgaattatataagattcttcacaaaacttggttacagtgtacattttacacctctttttttacttaaatgtatagtaaactaaaagg
ttgaactcaatggatatgtatcttatcatccttaaatactatgttactatgtttatatataatgaaggcttctaaacataaaggatcactatcaccagac
agtgacttctgcaatatttagtcaaggcttcaaaaaagtgaatttcctgtgatttatcacattggattttcatagaatgccagcagtcttacttctctgc
aaaacaacagcacaactatcttttggtatcagttttgtcaataaaaatttcttctggagaggtttggtgacaaaattcatttgcattatcagtttcctat
aataatcagtgtgcactataataactttcaattggattttcaaataatgtttgatcttatgtgccattatcactccagatttgtactcataataattgga
agttattagtaaaaattcctttctctccccataactttcagatacacttaaggngggcccgattttagctgccgatatcggtcctttagaccgatacaga
agcttatcatatggcctgaaatcgtccggatcacgatcgggcagggttgatttttccatcagatcagggactgcataggttcattgatgcagtgcctgaa
acgacagtacctgccactttaaatgcgatgtt
3' EST: CX982967 [+]ttttttgagaattgaaggtattttattaaaagttgttgaagtccttttctgtgtatgttccacagatttaaggtttgacaaactgtatctataaatagtt
gagtcatgttaaggttttggctcattttctttagggtcttgttcagctcctcaaacttgtgaatctgtcgtatgaagaagtctccataccgcctagcaac
tttagtatctgcaatgtgtatcatatccttatctatgtagaagtctacttctgaggcagactggaattctccttcgagggcagaaataccactggtccaa
accaagttcttcattttgtgtttgaaaaccagtaactgaatacggaactcttgttctgggagatccaagaatccagccagtttggctacaggcatggttg
tatagagtttcagaaaacttcttattgtggaaagctgggcttgctgctgcacctcatcaaggaacaccttaagttgctgcaggtagggctccttgtggta
gttgggatgaacgttgtcataattggggaccactggggacaggaatttggggcaagcataattgaaaagctcctcatagatctgggcatctcctttctgc
atgcgaagcatcttatccccatatttctctcttagctgagtgtggatgctctcgtcaattctcatggggtacatggtgagggcgatggctagaagaccat
gcatctgctcattctgcttgttaatcatctcatacttgtatgtggtcctttgaaacatactcttcgtcctctgaatatacagcagaatgttggcaaacac
acggatggcatcctgatatctcctcatcatgaggtaagcaaagcctacatagtaatatgtagtgacctggcactcaggcacacgtgaatacatgctcttt
ttgtaagctcaatatctccagcaccttgatgg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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