XB-CLONE-3539155
Clone Name: IMAGE:7719444
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Gene:
cdo1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX982976 [+]
caacatggagcagactgaagtgctaaagcccaacaccctggatgagctgatccagatcctgcatgagatatttgccagtgataaagtgaatatagaggag
gtgcaggctatagtggagtcctatgaaagcaacccaagggaatggatgaaatttgccaagtttgaccagtacaggtacacccgaaatcttgtggatgagg
gaaatggaaagttcaatctaatgatcctgtgctggggagaaggacatggcagcagtatccatgaccatgccaactcccactgcttcttgaaaatccttca
gggaagcctcaaagaaactctgtatgagtggcccaagaaaaaaagtaacactgaaatggtgaagaaggcagaaggtgtattgaagctgaatcaatgtgcc
tatattaatgattctattggcctccatcgtgtagagaacccgagccatacagagcctgctgtaagcctccatttatacagccctccatttaatgagtgcc
acacattcgatcagaggactggacacacaaattcagtgaaaatgactttttggagcaagtatggagacagaactccttttgcaattgcacagtcgcagga
aaataactaaaccggatccaaaaaaatgaataacctgagaaactggaaatattttagattcactcaatgctgatgtgaccaaactgtaactggacctcct
gggaaacttgt
3' EST: CX982975 [+]gacaggtataggatctagtatctggaatgcttgggccttgggtgttagataatgcctcttgcatggagaattatgctttaaaaactactatgattattgg
tgccattgctccatgctggcttatttaacatcaagtacaaggtactgtcttgttattattgacacaaaaaagtaaaagtgaagtattaaatagcaatgct
gtgtttcagggccaatctgggcttccagataatataccatctgtatgattactggagtcaacacatttttattgttttaaaagccacacgctcacatgaa
acatcaaagcaaagttgctaaatgaattgctcaatcacggtaggaggaggattgttttccttattgttggataagtcactacaacaatgataacttatct
tatcaaacttaacatgaacaacagtacaaggaaacagactcataaaagagacttaaaagaacacggatgagcaagccaattcatatgctagttcttctaa
aaatattaagatcagaaaatgtacatttgttacaacaaacattaaaaactatacaattattctagaaaattcaaggctattcagcagaaattgtttcaca
ggaaagcagctttacaaaaattaaagaatattccagatttccagttttaaagcagatgtttcttaatttgcattactgtacagtttatttcaaaacaggg
aattaaaatgttgatttccttaggtctcaagaaatgccgccattggatgaaagtttctgccttttttcccacaagtttcccaggaggtccagttacagtt
tggtcacatcagcattgagtgaatctaaaatattttcagttttctcaggtattcatttttttggatccggt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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