XB-CLONE-3539152
Clone Name: IMAGE:7719492
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Gene:
apob
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX982978 [+]
caacctcattttaatgatgattttgcataatgcaaacaatgaaatgataatagttaaaaataatgccatcctataagaaacaatctttttattagcttac
aataacgaaaacaaatttttgaattgttcacacaaaaacacacatttgagggacgtgtaaaccggttgttgctactgtacttgcaaacaacacaaaagcc
tacgtttcagctatgccataatatacagcctgctcagttttttttgccagttccgaatgttctcaatgatctgctctgcagttagtttcccttcttgaag
ttgtctctcgataaactgctgcagctcttcccatttctgagaacctttgtcgatgccatccagtaccatggcgcgcgcaatttctgctcgtttgcttata
atgtcctctcttaaatgcgggatttcatcgaaggatttccagtcaaagggatgagggacttcaaccttaagttctcctttacgtgtggatatataggtct
tcatttcttcagtggccgaattctgaatgctttggagaagctggttgacatactctatgaaagagttgtagttttggatgataatgtcaatgagtcggct
tgcctcggcaataagcttctcgtatgaatttacaaacaggttagatgtagcttgaagtttttcattaagaatcttattggcattttgtgaaagttgcctg
gcagttgcagaccacttagttactctatcttcaagatctattgaagccctccttatgagttctttgccttgtccattagcattggtcagcaaatcgttag
catgttgggtatattttttaacaaagtcctttgtttg
3' EST: CX982977 [+]cggacgcgcaactggaacaattcaaaatgctctcacatttgcggcaaagccatttgatataaacctgtctactacaaacaatggaaatctgaaagttggc
tttcctttaaaactcactggcaaaattgagttcctgaacaactacaactttatactcaatacaaatgttcagcaaatatcttggcaagtcaatggcagat
tcaatcaatataaagtttcacatacgatttccgttggcaataatgaagactctgttgtggccattgtaggaatgaatggtgaagcaaaccttgattttct
gaccattccaataagcatccctgaaatgaatgttccatatacgtcaatgaaaacacctgaagtaaaagacttttctctgtgggaacaagctggtttaaag
aaacttttaacaactaccaagcagaatgttgacttgaatgtgaaacttcagtacaagaagaatatggacgtgcattccattcagttgcctctagattcct
tatatgactccataaattccaacatgaaagttcttacaaagaactttgagaagggaagggacaacacactcagaatgctcacggactcctacaatgaagc
aaaagctaagtttgacaagtacaaggtggaaaattcagctggtaaagcaccaagaaccataagagtacctgggtacaccataccactcctcaacattgaa
gtttccccttttagtgcagaacttccggcatttggctatgtcattccaaaggaaatcagcacaccaagctttagcttgcctgtgatgggattctccatac
caacctacacagtagttttgccatcattggaactccctgtccttcatgtaccaaatactctgcgcnaacttacactccccaaattgaactgccagcgggc
cacacaccatttctattccagcat
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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