XB-CLONE-3539128
Clone Name: IMAGE:7719590
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Gene:
thoc5
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX982994 [+]
caccagcagaccttgtcacgtctggactgggaactggaacaaagaaagaggcttgcagagaagtacaaggagtgtttggccagcaaggagaagatcctaa
aagagattgaaattaagaaggagtatcttaatagtttacagccacagcttaacagcattatgcaggcctcacttccagttcaagaatatttatccatgcc
gtttgactgcatgcataaacaatatgagacagcacgccaccttccaccaccactatatgttttatttgtccaggctagtgcctacagccaagcttgtggt
aagtatgctgggtttgcataagagtgaaagaaaatgactaaaggatttagagatgctataggctttgttagtgaataattataattgatgttaccatatg
gttaaagccagcaatgccatattgcctggcaaatttcagaaactacaatgtcctttttacagcgttgttcccagttgctgccagctatgtctaattaagc
cctgcttaacttctttaattggttgactttatggctcatggcagaaaatcaacatattttatattttggggaacaaaagttgctttaggctttattttct
gtaggctacctaaccagaaaaaacattggttagggtgaaaaggacaaaaggagacaaagagcttttctcaaatgcaaacatgcacccaagagcctcctta
accagaaggcattcacatgctttcatgctacacgcatagcactgccttaaaaccacaaatgagtgactgtaacctccactncaggaaatataggtggaga
catgcaaatgactccttaatgtccctgtggccaattatgcatcctgaattggtggtctaatagcacagatacagcctaatattttagagcgatatttagt
tt
3' EST: CX982993 [+]gggcataagtccaagcaacagtggtggcagagaaaatgctgaaagagttggaactcagcggtgactaaagaatccttgagggtaattatacttgaatggc
ttggtacgactcggccccctgagtaggcgcagacagatcttttctggaggaaactcatgaggcccttccacactgttgtcatggcgttcagtttctaggt
agacatccaacagcatgcaaagcctctgaatttggtttgtcagcagctggaatcctggaggagggccacacagttccttgaagtagacattgacctcact
ctccataaccctgatattgtcatcattactggatgagcgttctcctttccagtttaggcagaggctaaacactggaggtacagcacaataaccagggttc
agaaccactactccattgagcttagctgttcctcgctcaataagcagatagaaatactgatccgtttcctttgctagtccacactctataacatcttttg
tatacggcagcgctagataatcctcataggtgatgacattccactttgtcagtcgagatataaccttggctgggaaaagagtttggcactccaaagatac
aggaatgctaccatgttccaaggaggcaaactgccggtgcaaagacaagcgtgaaagcaatctggctctcagcaagttaatagtcttctccatgtgacta
gcactgagagcattgtctgctatgactgcaggctgcggctgatccgttggaaaatgaagtcctcccattgtttggacccatatatatgggtgtcccagtt
cagatatatagtcattaagggacaagattccgattttatcaaactggtatctgtttgctggatttggagtggtctttccatcatccc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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