XB-CLONE-3539076
Clone Name: IMAGE:7719690
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Gene:
rpl7l1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX983030 [+]
aaaagttgcaaaacaataaattgagggacttccagcgagagggagcgggtgtcacgcgggcaggttgggcccaactgtgtggcccttggggtacaggaac
cctgtgtcccctatccactgccctctccaacaccattggctaagggatcagtcacatgatgttcctgtgccccagggggcggccagagacgctctgtagg
tgaagagacagccgtgagtggaacgcagcaatctgagagcaggggctctagttcagctggcggatcagctggttaatgccggagcccctgtggccggggt
cgcccacctcgctcaggtagcccttccgattgacggctgcatggcgcgacaccgagaggcggaaggggcacaggaacctgttgacttctgggaaatgttc
ccccgttgtgtataactcatggatcacgtcctccaaacagatgatcccaaacttccccagttgctcctcaatcacgttgttgtcagtcagagccactcgc
cggccgtcccttctgaaatggcccctttttaagaccaactctctgattgatttcagatttgggaacccccacgcaacgtacggttctacaactcgaatca
ggttgagggatgagggagtcaacttcacaaaagctcctgcaaaaatcttccccaagcgaagggtctttagagcgtccagaaccgcgttactgactccatc
gacttttgtaatacgaaccacaaacgccagcgactgggtggcgacgccaggaatcttcttctgataaatcttcattctcttcagccgagcgtcgtccctc
agtttcctgtgagagtttcgtacgaaggtttccaaacgcttgaacttgatttgcttcccaggctgtgtctttcgcttctcctccacgctctcttggcctc
ggctgctttcag
3' EST: CX983029 [+]aaaaagtatctgggggctgaaagcagccgaggccaagagagcgttggaggagaagcgaaagacacagcctgggaagcaaatcaagttcaagcgtttggaa
accttcgtacgaaactctcacaggaaactgagggacgacgctcggctgaagagaatgaagatttatcagaagaagattcctggcgtcgccacccagtcgc
tggcgtttgtggttcgtattacaaaagtcgatggagtcagtaacgcggttctggacgctctaaagacccttcgcttggggaagatttttgcaggagcttt
tgtgaagttgactccctcatccctcaacctgattcgagttgtagaaccgtacgttgcgtgggggttcccaaatctgaaatcaatcagagagttggtctta
aaaaggggccatttcagaagggacggccggcgagtggctctgactgacaacaacgtgattgaggagcaactggggaagtttgggatcatctgtttggagg
acgtgatccatgagttatacacaacgggggaacatttcccagaagtcaacaggttcctgtgccccttccgcctctcggtgtcgcgccatgcagccgtcaa
tcggaagggctacctgagcgaggtgggcgaccccggccacaggggctccggcattaaccagctgatccgccagctgaactagagcccctgctctcagatt
gctgcgttccactcacggctgtctcttcacctacagagcgtctctggccgccccctggggcacaggaacatcatgtgactgatcccttagccaatggtgt
tggagagggcagtggataggggacacagggttcctgtacccccagggccacacagttgggccccacctgcccgcgtgacacccgctccctctcgctggaa
gtccctcaattta
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: