XB-CLONE-3539073
Clone Name: IMAGE:7719738
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Gene:
hnrnpdl
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX983032 [+]
tgcaccgatcacttcacaagcacaaacaaggaacttaaaaatattgctgaacataaaaccctctttcatacagatggcaaaaaaatgccattgaaataaa
aaaaaaaaaaaatcccagcttctatttacagcaagacacttagtgcctcagtcatagtcctctcaaaagagtaacatgatggcggctcccggcattgtcg
caggatgttaagtctctttatccattgccacctgaaacaaagaaaagagaatccttaattttcaatcagaagtcatccacttcacgagcccacatttcta
caaagtaaaaatcatatgcacattaaatgctatgtacagtgtgtaaaacctaactttagtttgacatttatcgctgatatatccaacatctctgaagctg
taacctcagaagtggtcaagcttgtaaactgtataaaggattcattaggtctgtgcataggattttttttctttttaaatttacaagacatatcacccct
cattttaacaagttcttgtttgtctgtagattttttagctataaagaaaagaaataagcattaaatcctataaacgcacaatttaggaatacaattaaat
taaaacgaagagcaaaaaagattaagctgtaaaggcatctaactttttaaactcctgtaaaaaacaatttatcaaaatgttaacaaaaaaggactccaaa
agattaaaaaagccaaagcctgtagttgtttttcccagtttaatttttacgctggtacatggaagttggaagagaccataccacaggacagcggtttctg
gtgtgtgccttgcgctctgcccacaacgtgcga
3' EST: CX983031 [+]tggtgccaccccnggacttcaacgaaggctcaaaaatcaatgccagcaaaaaccagcaggatgaaggcaaaatgttcatcgggggtctgagttgggatac
aagtaagaaagaccttacagagtatctgtctcggtttggggaggtggtagattgcacaatcaaaactgatccagtgactgggcgttcaagaggatttggc
tttgttcttttcaaagatgctgtgagcgttgacaaagtgcttgaaacaaaagagcataaactagatggcaagctcattgacccaaaaagagctaaggcac
taaaaggcaaagagcctcccaagaaagtgtttgttggcggactcagtcctgaaacaacagaggagcagatcaagcagtattttggtggatttggagaaat
tgaaaatattgagctgccaatggacacaaaaacaaatgaaaggagaggcttttgttttgtcacctatacaggcgaagaaccagtaaagaaacttttggaa
agccggttccatcaaattggtacaggaaagtgtgaaataaaagttgcacagcccaaagaagtctacaggcagcaacaacagaagcagcagaagggaggaa
gaggagcagccactggaaggggtggtgccagaggaaggggcagaggtcaaggttggaatcaaggctacagcaactactatgatcagaactatggaagcta
tggaaacaatggttcttatgctgatcaagggtataataacagttatagtggttatgattactctggatataactatggaagctatggatataatcaagga
tacacagactacagtggg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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