XB-CLONE-3539061
Clone Name: IMAGE:7719596
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Gene:
tfg
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX983040 [+]
cagaaggaggagaagtggtattagtactgccgccctcaccctgtctccttggcatttaatcttacggcggtcggtaaccagcccggtatatctacggggg
agcgaacagcagcgtcagctgcctaaatcaccacggagcccacaatgaatggacaactggacttaagtggcaagttgattataaaagctcagttgggaga
agacatcagaagaattccaattcacaatgaagacattacctatgatgagctggtgctgatgatgcaaagagttttcagaggaaagctccttactaatgat
gaggtgaccatcaagtacaaagatgaagatggagatcttataacaatatttgatagctctgatttgtcgtttgccatccagtgcagtagaatattaaagc
tcaccctttttgtaaatggacagccaagaccattggaatctagtcaagtaaagtatctccgccgggagttgatagaactacgtaacaaagttaatcggtt
gctggactgcctggaaccacctgcagaacctggaataccgtccagccttcccgacaatgaagctgtggatggcagagaagagaaaccacctgcaccagat
tcctctgttaaaccatctacccaagttatggctgccagcatgtctgcttttgatccactgaagaacccagatgaaatcaacaaaaatgtcatgtctgcat
ttggactttctgaggagcaagtcactggacctcctagcgcaccagtagaggaacgttccagcacgccagatagcattgcttcttcctcctctgcacatcc
accagggactcagactcagcagccatcatacccaggagcccagcagcaagcaggtcagattgaaggacagatatatcaacagtatccacagccacagcag
cctgctgggtatccagctcctcaag
3' EST: CX983039 [+]ttaggacagatctaatttaacacatcagatcattaaaaacaagccaaataatgggggttgccatttgcattgtgtcatagttttgacaaggccggctagt
gtcaaacacatttttgtatttgaatgtctactatagagagcaggtactatcaaaagcagaaaagaataatttggtctctacttgaattaaattacacaac
atttagtgaaaagcaacagtgtgctgagaagcacactaagcttttcctctgatgtctgaaacatcagtacaatttaagcataagattggaatctattggg
atggatatagacctagcagccattgccttggcagtaatagtgagagtgacatttaccggtaaccaggtcctggctgagcgtatccctgaccaaatggtag
gcgattgcgcgcatatgggttagggccacttgggggtggggttacagtacttccaggggattgtgtaaatcctgccctaggctgatagctccctggttgg
cttggagccagtccaggttgggaaacaggaggcggattgtagtttgcaggctgagaggcttgtgtagtgtaagtctgtggagggtatcccccagcctggt
actgctgagggggctgtgaagccatttgctgttgctgccctgggaaccctggagctggagtctgtgctgcttgctgattgtaagctggatactgagctgg
ttgaggagtagcttgctgattgtaaccagaatactgcatgccgtagggttgctgaggctgggctggagcttgnggctggccttgaggagctggataacca
gcaggctgctgtggctgtggatactgttgatatatctgtccttcaatctgacctgcttgctgctgggctcctgggtatgatggctgctgagtctgagtcc
ct
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: