XB-CLONE-3539044
Clone Name: IMAGE:7719646
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Gene:
myo15b
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX983054 [+]
ctgatttactttgaagacaaggagctgaatgagccggcggctaataatttcatgagtctgatgagattcatgggagaccaacagttcagacagcaggatg
atatgggatgtgcatttgaaatcctacagctatgccgggagaaacaagtgctgagagatgaaatctactgccaagtgattaagcaaatcacagcgaaccc
caaacaggatagctgtaatcgtggctggcgtatcctaagcctcctgacgggattctatctgccatcacccaacttactgccatgtgtcaccaagtacctt
caggactgcgtcggcgactaccaagaagtgtcccggctctgccaggagcaccttcgccggaccgtgctgtaccatgggcgcagacatcttccatcaaaag
ccgagatggacgctttgctgaatgaccgagtatctcgccgactgattataaagttacccggaggagctgagttcaccaccaaaattaagaccttcactgt
ggcggctgacgtggtgcccgagatttgtgagcaaataaacgtctctgagcctgaggagaccgatgagtttgctctgtttgccaacaagaacaaaggtgaa
gtggtacggcctctgcgtgggggcgaatatatccacgacttcctgctgcgggacaactctgtggacctggaattccgccgagtgacttggaaggccaccc
tgagggggcgcagtgagctgtatatccaattgcacttcagtcagatgctcagtgactacatgcagggcaaagccttgttactgagcccatccgatcttct
gggggtgcagacggggcgagtggctgccattctgcacaggataaagggccttagcagccccccgagcaagc
3' EST: CX983053 [+]attctctattttctctttaatggttcattatttaaatacagggatttattagcacaattgcacggcgatatcagcatcacaaattataggaataatcagt
atctgtactgatcctagcccccgtgccccctaaagcccccatgtaagagcagagcaacagcatggtaggggggggaacttcatgtgctccttggatacaa
acccactgacatgaatgggaagtcccttgggccacccctacagagaccacccctaagtgcacatgggggcaatcccagtcccagatgattttgcgctata
ttgttcttggcaccggcaaatcaaaataattagaatgcaaataagttacaagggcagagtttgggctcccggggtcctatgttggcgcagtctccatgtt
ttctatgaccaaggccagtgtgtgatacagctcttttgcctcctggagttccacccacatggtacgagggtttgcggccgaaccataattaatttccact
cccggtagcgtgttgctgtcaaaggggcgcattgtgcgcatgctctgaatatcttgcaggttgatacacatgcttggttcttgactgttcttctccaaga
ccagtaaatgctggtgattgatggccacaaagcaaggggacgtcatgccgggttcacttattcgcgtcacatggtatacgttgtattcaaataggggcag
aatggaaaccgtttccaagaatcggatctttgcctgatgagcagagatgttctgggtctctctgagctcctgaagaaggctctgctgtgcagcctgaagg
ttcaggcggttgtgcactgagttgggaatataatcc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: