XB-CLONE-3538993
Clone Name: IMAGE:7719556
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Gene:
eps8l3
,
tmem98
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX983089 [+]
acgaggtgggactttagtcatttgactgatagtgaaatggagaccgtggtcattgtggctattggtgtcctggccactatatttttggcttcctttgtgg
ctctggttgtggtttgcaggcagagatactgcagaacaaagaatctattaacacattacaataacaaaccaacagtggatttaattggagccatggaaac
acagtcagaaccctctgatctagagctggatgatgttgtcatcacaaaccctcacatagaagccatcttggaagacgaggactggattgaggatgcctcg
taatttttcagataacattgttcttttacttaatggttttacattcaagaagatggttttatttcgggcagatgcaatgcattgttgttgctataggatt
ctgtatttatatggaagcaaacatgggacttttgggctaagaacagtttgtaaaacataaagaaataccccagaaaggatagggtaaaatctcaccagtg
gaaacattgggtggcaagctacaaaccctcagctatgggagcggaacaccataaataatgtagaagaactttaatggaaacctagaaagctgggtgtgat
tacttctcccggtgaaaattattttgtagctacatatttatatatcagttaaaatgagacatgtccgtcaaccattttctaaaatcaattgtcgccaact
tcttgcttgggattcttatccaacagattggaaaccaacattgtgggtctccttgttgtttagattgngtctccaagatccgctttaataatatgacgtt
tctaaaccaatgtttgggtcacaaagctgattcgttcacttaatgttggtcccctgagaa
3' EST: CX983088 [+]tatagggcgagaggtttaagtccaaggatcaatcgaaaaaaaactgtgacacaaagatacagcttcactccacatatttctttttggcggtcctcacaga
tcgagtgtgaaaatgtattgcaacttctcaacaatcccatagtggtttcatgtctcaagagatcctctcactgtgctcagttgcaaatatacacgacccc
cttcctcgggacacacagttttcaagtcttcacgagttagctccaaaagctgatctccttgaagaactccaagacatctcacagtgatattggagaagcc
tttctgttgaagccacgctttcacctcctctggacgagaacttggatttagtaccactgaacttgcaggagcctgaccatcatctgtgttctccaggatg
ttgcttggaataaatccctgctggccctgagaattctgcaccatccaccactgccggctctggtccaaaaccttcacaatgtctcctttccttactgaca
gttcgcgattatttctcgcttcaaagtcatgaaggacattcatgaggagtgggtgaaattgcctgtttcgtagtggtgtttcaggactacgtgacatctg
ggatgaaacatcactaaaggggatatcagggatgatccacccatcagaaaaagttggaacatattgaggtatattctttccatttggccattcagctctg
gtcttcatccaggcatctcccaggctttcccagaattttctttctttgtctgtcacacaagaactcataatcatcagtgccttctgtgtaagcagtggag
ataacacagactgtggcaagtctttttgtggacacctgtctacaatcttggccagggtttccatcatcaaatggatgagatctggagcacttggttgttt
cattttgtcgtggacattccccat
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: