XB-CLONE-3538905
Clone Name: IMAGE:7719612
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Gene:
mhc1-uaa
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX983149 [+]
ctcagcactcatttttcaaatttagaaattcctgaacaaaccccttccttactgcttctgtttccaggcatgacaagttccaggattgtagaactggaaa
ctttggttctctgcgtctctatacagttgtcagtggattggagggaactgttgttcagtaactggtccaggataaaagtaaatgttccctactatggctg
acattatgaaagaatgaaaacccttgagaaaatttatagctggggctgatgccacctcttattcaacttacaattcaaaagctggggggttgaagcctgg
gtgctcacatgtggtacagaggtcacagtgtgggtcatacagtggtgttgttctggatatgaaccagggcagaactaagggtaggcagaagaagcatgtg
cctagggtgggggctgtcttgcagaccctgcaagagtgtagagggcttagtagggctctaagtaatgagtgacttcaatagaccttagtagaatagttaa
ctcaccaatgtctggggccctacactgaatttgctgtggggcccagtaatatgtagttatggcactggaaatgcccatcatgatgggagacaccatgtca
ctttcagtggaaccacatatagcggtcataacacaacagtgattggtctaatgttgtctaaggaaagtatcctcataaagaaagcaatatcaaagttcct
cttatgcagaanatttatgttttaggaatcanagaaaatttccaattaacatcagagtacatttttctttgaatagaaaccttaaccaagagcctttctt
gtgaacaggagtagaagcaacagtttcattgaatttaaaatttgcctgct
3' EST: CX983148 [+]agcaggcaaatttttaaattcaatgaaactgttgcttctactcctgttcacaagaaaggctcttggttaaggtttctattcaaagaaaaatgtactctga
tgttaattggaaattttctttgattcctaaaacataaattttctgcataagaggaactttgatattgctttctttatgaggatactttccttagacaaca
ttagaccaatcactgttgtgttatgaccgctatatgtggttccactgaaagtgacatggtgtctcccatcatgatgggcatttccagtgccataactaca
tattactgggccccacagcaaattcagtgtagggccccagacattggtgagttaactattctactaaggtctattgaagtcactcattacttagagccct
actaagccctctacactcttgcagggtctgcaagacagcccccaccctaggcacatgcttcttctgcctacccttagttctgccctggttcatatccaga
acaacaccactgtatgacccacactgtgacctctgtaccacatgtgagcacccaggcttcaaccccccagcttttgaattgtaagttgaataagaggtgg
catcagccccagctataaattttctcaagggttttcattctttcataatgtcagccatagtagggaacatttacttttatcctggaccagttactgaaca
acagttccctccaatccactgacaactgtatagagacgcagagaaccaaagtttccagttctacaatcctggaacttgtcatgcctggaaacagaagcag
taaggaaggggtttgttcaggaatttctaaatttgaaaaatggtgctgag
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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