XB-CLONE-3538848
Clone Name: IMAGE:7719666
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Gene:
exosc4
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX983192 [+]
attcagaaggccgcgactgcgcaacattccgttcctatggcaacaaacatcaatgtattattacacgtgcaaaataacagcaactgggttttgccccatt
ggggctgcggaacactgacagataggtaagttgggggcaaaacgcacatgttggagacggtattgtaagcaataagctaaacctgttacccagaaggctt
agggcctgggggtttctggataaggggtctttctgtaatttagggtttgttcacctttgagttaacttttagtatgatgcagaaagtaatattctgagac
aatttgcaattggtcttttttattattattttactttttgttccgcagctcctccagtttggagtttcagccgttatctggttgctagggtccaatttac
cttagcaaccagggagtggtttgtgagagtgactaatatatgaataggggaggggctgaatagaaagagaataaaaagtaacaataacaataaaactgga
gcctcacagagcaatagggtttggctgccggggtcagtgacccccatttgaaagctgcagagtcagaagaaggaggcaaactataaaaaataaataatga
agaccaattgaaaagttgctgagaattggccagtctataacatactaaatgttaagttgaaggtgaacctcccctttaaaatggagaagccgaagcagcg
gtgggggtcgacccccctttatactataaacccccatattattattaagaacgaacccctcggatatgtacaagtaacaacaagtgcaataataattaca
atgatcccttattacattcagtgcagcctgtatatcccgctattacatacctgtgtgggcttggcgctgtcctttggc
3' EST: CX983191 [+]ctgtggcaccctgggaaatgaagaatatggctagccccatgggaaaaacagattacaatgcaggattctgctggagtattgctttacattgctcagggag
gggggtatctaacctgcttccctgcagttttattgctcagtgttacagcccccccagcagggggcaccccaggccccagtcactcattcatgatattggc
agtgtaaaaactattatgatgatgggattgaaaaaaaaaatactgtataatatgatattatacaggggggtaaatatctgcttcactcattgcttccggc
ttccgaccaattacacggctgaagccaaaggacagcgccaaagccacacaggtatgtaatagcgggatatacaggctgcactgaatgtaataagggatca
ttgtaattattattgcacttgttgttacttgtacatatccgaggggttcgttcttaataataatatgggggtttatagtataaaggggggtcgaccccca
ccgctgcttcggcttctccattttaaaggggaggttcaccttcaacttaacatttagtatgttatagactggccaattctcagcaacttttcaattggtc
ttcattatttattttttatagtttgcctccttcttctgactctgcagctttcaaatgggggtcactgaccccggcagccaaaccctattgctctgtgagg
ctccagttttattgttattgttactttttattctctttctattcagcccctcccctattcatatattagtcactctcacaaaccactccctggttgctaa
ggtaaattggaccctagcaaccagataacggctgaaactccaaactggaggagctgcggaacaaaaagtaaaataatata
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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