XB-CLONE-3538839
Clone Name: IMAGE:7719810
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Gene:
crybg2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC125761 [+]
GGAAAAAGGAACGGAGTGGTGCAGAAATCGAAAGGCAGGGATGGGGCCATAGTCTCCCACACCTCAGATACCACAGAACTGAAGATTCCATGTTGAGAAG
AACCAACAGAGCATCAAAGCAACATACCAAGAAAGAACATGGCAAAGTGAACCCCAGACCAGGGAAGGTTCAACTGTTTTCAGAATCAGGGTTCAGTGGG
AAGTACTATGAAATTTATGGAAATGTGAGTGACACAAGTGAATGGGAACTTCAGGAAACCATATCGATACGGGTTATCCGAGGGGGATGGATCCTGTATG
AAGAGCCAAGGTTCCATGGTAGGCGTGTTATGCTTTTAGAAGGTGATGCCGAACTAAAGTGTCCATGGCTAGGGCAGACTGACTCAAACCTAGAGCCCCA
ATTAACATCAGCATTTTGGGTTGGATCCCTGCGCCTTGTTGTTAGGGATTTTCGGGTCCCCAGAATTAGTCTGTTTTTGAAGCCAAATGGGGAAGGAGAG
AAAGTACAAATTGAAGGAGCTGCACCTGATATGAGTGAATATGGTGGGGCAATCAAAACAGAATCCATCATTGTGCACTGTGGCATGTGGCTTGTATTTA
GCAAGCCATTATTTCAAGGTGACCCCTACATTCTGGAACCTGGTGGTTATCCCAACAGGACATCCTGGGGGGCAGAGGATCCTCAAGTATGCTCCCTTGA
ATCTGCACGTATTGGAGGCCCCACTGTGGAAAAACCTAATGAGCCCAAACTCCTCCTGTTTCAGAACACTGGATTTGAAGGTACATGTTTGGAGGTGACT
AGAGACATGCATTCTATTCAGGGGGAGCCAAACCTTCGGGGTGAACAACTATATTCTTTGGGATCTTTGAAGGCCTCGGGGGGCTGTTGGGTGGGGTATG
AGAAAGAAGGATTTCGAGGTCACCAGTACCTGCTGGAAGAAGGTGAATATAATGATTTCTCTGACTGGGGTGGCTGCACAGAGGAGCTGGGATCTGTCAG
ACTTATTCGAACGGATTTTTCTCAACCTGAGATTGTGCTCTATGAGTTGCCTGGATGTTTAGAGGGTCCATGTTTGCGCCTTAGTGAATCCTTGGCTGAT
TTAGAAGAGGCTCAGTATGGAACAAGCACGGGGTCCATTCATGTTCTAAGTGGAGTGTGGGTGGCATACGAGAATGTGGATTTCTCAGGGGAGCAGTATA
TTTTAGAGAAAGGGATCTATCACAACTACCAGGATTGGGGAGCTGAGAGCGGCAAGATCTCTTCTGTGCAACCAGTGATACAGGTTGGGGGAAACAGTCT
CCATTTTGTATCCAAGATCCACTTGTTCTCGGAGCCAAACTTCTATGGTGATTGTTTAACTTGCAGTGAAGACCACATGGAGCTCCCAGAATACTTTTCA
CCCCAGTCTTGCAGGGTGGAGTGTGGAAGCTGGAGCCTGTATGAGGGCAAGAATTGCAGTGGAGAACAGTACATTCTGTCAGAAGGAGAATACCCAACTC
GCACTGCCATGGGGTGTCTGACACCTTGCCCTCTACGATCTTTCAAGATGGTGCCTCTGTACTTTTCTATTCCTTCTATATCGCTCCATGGTTTAGAGAG
ATTTGAGGGAAAGGAACTGGATTTTACGGAAGAAGTTCGGAGCCTTCAGGGAGAGGGGTACAACAACCATGTGATGTCAGTGAGCGTAAAGAGTGGCATA
TGGGTTGTTTATGAACACAGTGATTTTAGGGGTAGACAGTGGTTATTGGAACGTAAGCAGATTCCTAACTGGCTGCTTTACAGCGGGCTCCAGAGAATTG
GATCACTTTGCCCCATCCGACAGAGGACTGTGTATTTCCGTCTTCGTAATCGCAATCTGGGCCTATTTCTCTGTATTGCTGAGCTGACAGAAGATATGAA
GGCAGCCCGGGTACTTGTGACTGAGCCAGAAGATGGGAGGTGTTTGCTGTGGTACTACGAGGAGGGACGTCTTAAAAACCAGATGGCTCCTCATATGAGT
TTGCAAGTGATTGGCCAATCATGCTCTGGAACCAAGGTGGTGTTATGGTCTGAGAGTCGAAAGCCAATTCAGACCTGGATCCTAGAAGATTCTGGTTGCA
TAACAAGTTGCATCTTTGAAGGAATGTGCCTTGATGTAAAAGGAGGCCAATGTTATGACTCGGACCATGTGGTAATATGGGAGACTGCAGAAGACAGACC
AACCCAGCAATGGGACCTGGAAGTGTTCTAACAAAGCAAAGTGGACCCAAAGCAAAGAGGATTGTGAAAATGTTAGCAAGGGTATACGCAGCATAATTCC
TAAACGTTTACTTCAAAAAATGGCAGCTGGACCAGAAACATCTAAACATAGCACAAAAAGGCACATTTTCCTGTCCTCTTATGTGCGCTGGTTTAGAAGG
CAGGAAACCCAGAACTAACGGTTTTGTAGTATTAAAGAATACTTTTCCCTTAATATTTAAGCTACAAACCCACCAGAAAACCTTAACCTAGTTACACTAA
CCTTATCTTCATCTAGTTTCTGTAAAGCGCAATCACCCTCTTATCCTCAATAGGAAATATATACCCTCTGATGTGTTAATTAAATGTAATATTTCAGCTG
TAATTTTTATTATCATCATTAATCATCTGCCAGAAGCTAGCATTATAATTTTATATAGTATTGTTCTATATAAAACTATAACTATAAAGCTATAATAGTA
TATTTTGACCACTGCTTCTATTTGCAGAAAAAATGACCTGGATAATGCAAACACAGGCAGGCAGGTCTTTGGTTAAGAAACTACTCAATTGAATGTTTTA
AAGCAGTTCTCTCCAACTTATAATATGTAGTGGGGTGATTATTTCTCATAAATCATGGCCAAGAGTCAGATTGTATTAAAATAATGTTGTACAATGAAGT
CTGACACCCTTGAGCAGAGTGGGGGGGGACATAAAAACTCTTTGGAGGGCCACATCTGACCTGCCGGCCTCCAGTTGGACAGCCTTGTTTTAGAGGGACT
GCCTGTCACTATTCTGTGTGTACGTGTGTCAGCCATGGTGCTTTCTATGTAATGGTATGGTTTACCTTACAGTTAACCTTTAGTATGTTATACCAATGTA
TTTAGTTGGTCTTTATTTTTTATGGTTTTTGAATTATTTGCTTTTTCTCTGACTCTTTCCAGTTTTCAAAAGGGTTTCAAAAGCTATTGCTTTTCAAGGC
TACAATTTCACTGTTATTGTTACTTTTTATTACTTATCTTTCTATTTAGACCCACTCCTATTCATATTCCAGTTTCTCATTGAACCACTGCCTGGTTGCT
AGGGAATTTGGACCCTGGCAACCATATTGTTGCTAAAATTCCAAACTGGGGAGTTGCTGAATATAAAGCTAAGCAATTCGAAAACACCAAACAATTAAAA
ATAAAGACTATATACAAATTGTTGCAGAATGGTGCGCTCTACACCCCCTTCAACATACTAAAAGTTTATGTAAAGGTGAACAACTCCTTTAATAACATCA
GGAATGATAATAAGAGGGCAGGGGTGGTTTTATCTGCTATTGCCACCCAATGGAAAACAGCAGCAGACAAAATTCCCCATGTGCCTCTAGCCTTGCAACA
AATCTTAAAAAGACATTATAATTCTGCCTTAATCCATATGTAAGAAATGTGAAGACGCCGTGCAGCACAGGTGGACATCACCCTGTATATAGTGTTAACT
AGCATAATGATGTTTTAAGTAAAGATACACTTTTATTTATATTTGAAATGTAAATTTTTATACAGATTTTACTTTCTCCTTTTTAAGTTCACAAACTCTG
GGTGGTTTTGTACATTCTGTATTTG
5' EST: CX983198 [+]gaaaaaggaacggagtggtgcagaaatcgaaaggcagggatggggccatagtctcccacacctcagataccacagaactgaagattccatgttgagaaga
accaacagagcatcaaagcaacataccaagaaagaacatggcaaagtgaaccccagaccagggaaggttcaactgttttcagaatcagggttcagtggga
agtactatgaaatttatggaaatgtgagtgacacaagtgaatgggaacttcaggaaaccatatcgatacgggttatccgagggggatggatcctgtatga
agagccaaggttccatggtaggcgtgttatgcttttagaaggtgatgccgaactaaagtgtccatggctagggcagactgactcaaacctagagccccaa
ttaacatcagcattttgggttggatccctgcgccttgttgttagggattttcgggtccccagaattagtctgtttttgaagccaaatggggaaggagaga
aagtacaaattgaaggagctgcacctgatatgagtgaatatggtggggcaatcaaaacagaatccatcattgtgcactgtggcatgtggcttgtatttag
caagccattatttcaaggtgacccctacattctggaacctggtggttatcccaacaggacatcctggggggcagaggatcctcaagtatgctcccttgaa
tctgcacgtattggaggccccactgtggaaaaacctaatgagcccaaactcctcctgtttcagaacactggatttgaaggtacatgtttggaggtgacta
gagacatgcattctattcagggggagc
3' EST: CX983197 [+]caaatacagaatgtacaaaaccacccagagtttgtgaacttaaaaaggagaaagtaaaatctgtataaaaatttacatttcaaatataaataaaagtgta
tctttacttaaaacatcattatgctagttaacactatatacagggtgatgtccacctgtgctgcacggcgtcttcacatttcttacatatggattaaggc
agaattataatgtctttttaagatttgttgcaaggctagaggcacatggggaattttgtctgctgctgttttccattgggtggcaatagcagataaaacc
acccctgccctcttattatcattcctgatgttattaaaggagttgttcacctttacataaacttttagtatgttgaagggggtgtagagcgcaccattct
gcaacaatttgtatatagtctttatttttaattgtttggtgttttcgaattgcttagctttatattcagcaactccccagtttggaattttagcaacaat
atggttgccagggtccaaattccctagcaaccaggcagtggttcaatgagaaactggaatatgaataggagtgggtctaaatagaaagataagtaataaa
aagtaacaataacagtgaaattgtagccttgaaaagcaatagcttttgaaacccttttgaaaactggaaagagtcagagaaaaagcaaataattcaaaaa
ccataaaaaataaagaccaactaaatacattggtataacatactaaaggttaactgtaaggtaaaccataccattacatagaaagcaccatggctgacac
acgtacacacagaatagtgacaggcagtccctctaaaacaaggctgtccaactggaggccggca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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