XB-CLONE-3538790
Clone Name: IMAGE:7719528
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Gene:
slc22a4
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX983232 [+]
ttgctcagcgccagcatcatccccaatggcttcaacggtatgtctgtcatgttcctggctggcatccccgagcatcggtgccacataccggagagcgcca
acctgagcgccgcttggaggaatgcgagcatcccccggcaggacgggcaggacagtcagtgccggagataccggctggggaccctcactaactactcggc
tctggggctgctccccgggcgggatgtggatgtgactgggatagagcaagagccgtgtctggatggctggaactacagcaaatccctttacctgtcaact
atcgtcactgagtgggacattgtgtgtgaaaatgactggaagggaccattaacgacatcattgttttttgttggtgtgctccttggttcatttgtttccg
gtcaactatcagacagttttggaagaaagaagattctttttgtgaccatggctatacagactggcttcagcattgtacaggttttctctactagctggga
aatgtttgccattctcttcctcattgttggaatgggtcagatttccaactatgttgcagctttcatattagggacagaaattcttggcaagtctgctaga
atcatcttttctaccttgggtacatgcatattttttgcaattggctacatgatgttgccactgtttgcttacttcattagagactggagaacactcttac
tggctctcactattcctggtctattctgtattccactgtggtggattataccagaatctccccagtggctcatctgtaagggcaggatagaggaagcaga
agtcatcatccgaaaggcagcaaagataaatagagtcc
3' EST: CX983231 [+]gtatttcaataactttgaatttttttatttataaaaatgattacaaatttcagaatttacaatatatgtaagttttttttcacaacagtttcccttttaa
taaaaaaactacatgtgcactcaaaaaccaaatgaaattatgaatttatatgggtaagccacagcaaccaataaatgtctgcttattccaacttaattag
atcagtacaagctaactgcttattgggtgctgggtgaggcaaactttgccccaagtagtaaatataccctcccaataaatctaggaaatagtgagaaact
gtatgcaaatatcaccatgggtctcatactacaacacagacactttcatatcaaagtgagaaaagatctctgtaatgcaagttaaattaatcacaatgga
aagaagcaaaataacataccaccccacagacacaccaaaaccatcaccattggtgcagccaactgttccccaaacacagttagatgaaaattacctgtgt
ggagtcaaaggcttcaaggaattgaagcatgaacatgtgtatcaagttcaccttctagtaagccagtattatggcataacttacactatgtaggtccaga
aaagggtaactgtaaagctaaagttaggggattgataaaaggaaatttctaaatcactgaatatcacttggagttttgctatatgaatcataaagaaatt
aaagggtacaggatagctgtaaatcagccaagaagagatcttcacccaaggtgggtgactatggaaaaagaagactgtgagggagtccaccaagagaccg
ataacaactctgag
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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