XB-CLONE-3538714
Clone Name: IMAGE:7720109
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Gene:
fth1.1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX983282 [+]
cttctgcatgattcagaacagtaatagcacagatttattcccatcatcctcaagataaaaaggtgtcaacaggggcatgggagctatggtgtaatccaca
cagatagtaaagagacagtattttgaaacagagagctgactggttgtttccaccctgagagtgcgcttagctactctcccccagggtgtgcttgtcgaac
aggtactcgcccatgccattctgcggcaccccaaggcgcttcaggtttgtgatgtagtctccaagctccttcatggccttcacctgttcctccaagtact
cagattccaggaagtcgcagagatgaggatcagtcttgtcagatgccagcttgtgcagatccagtaaagcctggttcacagtcttctccagctgcagagc
ggcctgcatggcttccagggtgttaccccattcgtcacgctctggtttcttgatatcctgaaggacggcgcggcccccacgtttgttttggtatttgagg
aacttttcggcatgctccctttcctcatgactctgctccttgaagaacttggccacatggtggagtgccacgtcatcacgatcgaaatagtaagacatgg
acagatagacataggaggcatacaactccatgttcaccatacggttgatggcggcttcgcagtcgctgttgaagttctgacgcacctgggattgcatggc
ggcgtttcggtgtttgctggtggcgcanaaaagtcacaaaaaaaagagtaatgcaagagagagagagagattttggtctaaa
3' EST: CX983281 [+]tttagaccaaatctctctctctctcttgcattactcttttttttgtgacttttttgcgccaccagcaaacaccgaaacgccgccatgcaatcccaggtgc
gtcagaacttcaacagcgactgcgaagccgccatcaaccgtatggtgaacatggagttgtatgcctcctatgtctatctgtccatgtcttactatttcga
tcgtgatgacgtggcactccaccatgtggccaagttcttcaaggagcagagtcatgaggaaagggagcatgccgaaaagttcctcaaataccaaaacaaa
cgtgggggccgcgccgtccttcaggatatcaagaaaccagagcgtgacgaatggggtaacaccctggaagccatgcaggccgctctgcagctggagaaga
ctgtgaaccaggctttactggatctgcacaagctggcatctgacaagactgatcctcatctctgcgacttcctggaatctgagtacttggaggaacaggt
gaaggccatgaaggagcttggagactacatcacaaacctgaagcgccttggggtgccgcagaatggcatgggcgagtacctgttcgacaagcacaccctg
ggggagagtagctaagcgcactctcagggtggaaacaaccagtcagctctctgtttcaaaatactgtctctttactatctgtgtggattacaccatagct
cccatgcccctgttgacacctttttatcttgaggatgatgggaataaatctgtgctattactgttctgaatcatgcaga
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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