XB-CLONE-3538653
Clone Name: IMAGE:7720019
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Gene:
olfm4
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX983322 [+]
ttcataatgcaaaataaaaaaaaaagcatttaataaaacactatggaattacagtgctgataaatgtggtaaagattaatagttctacaaatatttagag
aaaacaatagaatgcagagtttcttatgtaaaagaaactgctgtaaatggagtgcaaaaaaagttaagttaagcctcaattttgacccaacttgccattt
gtaaacctgaatataaagtctgatttaagcctgattcaacaatggtttaaagacaagatcataggtgattaagtaagcatcattgaacataaagagcttt
tgatcattagggttatatgcaagactctgaacattttccatcatcttgtccagaattatgctcagctgtccttccttattggtatttgtgtcatacatgt
agaatatctcttccttccgagtattaagtgttctagtggcataaagaacaccacaaaccatgaaggcatttgttgctccaagcttatactgagaagtgga
ccaagtcttttccactgttaaggaggtggaattgagtttgctaatcacaatgtttccagagttttgttcagtggaataaattgcccagagtccattttca
tcactggctagatcaatatcttgccagctggaggatgcataggagaaacggttattgaaggaagcgtctggtagtggcttacgttctacaacattagtgt
ccacactgtatttgcagatgtctctgctgttgtaacagttgtaatacatagtgtggttataaactatcatacccccaccttgtccacagttggtatagtc
aatttgattgtatggcagattcttagtgagtattttatctgtagggcttttgtaaagcaagaggttatcatatgaagtgtaaaatct
3' EST: CX983321 [+]ggcaatatcaactcctgggtctcttgatgagcatggagtctgtcagtgttccttgactgtcccagatatgacctttcctgctgatcgaatggagattctg
gaaagagaaaacatgaaactgagtgccagtgtccaggaacagatcactaagatagaagaatacaaggccacactaacaggatgcctgcagaagctgacca
atctgaccaagaaggttgaatttatggaaatgggaggcatctcctacacagagcttgactttgagctgctgaagctagagattaaggaaatgcaaacttt
agtagaacaactcagattttctatcaatggttccagtgctttagtggagagtctctttgaggagatacataacatctccattatggtcaaccatctggag
tcatatgataaaaacaatgtgctagctgtgcgtcgtgagatagctgcccttcggaagcgcctggaagattgtgagaagaaccgtgttactccaacacaac
cttctatcagttatggaacttgcaaacatggaggattggcaaacatcagtaaaccttttgtggtgcagctgaactggctgggtttttcctacaagtttgg
aggatggggtaaggacacatttccaggagccaatcaagataatcattgggtagctcctctgcaaactgatggacggataatgaacattgtgagattttac
acttcatatgataacctcttgctttacaaaagccctacagataaaatactcactaagaatctgccatacaatcaaattgactatacctactgtggacaag
gtgggggtatgatagtttataaccacactatgtattacaactgntacaacagcagagacatctgcnaata
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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