XB-CLONE-3538636
Clone Name: IMAGE:7719973
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Gene:
fgfr1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX983333 [+]
cacgtgttcttcaggcgaggactctgtgttctctcatgaccccctccctgatgaaccttgccttcccaaatactccaatggtggacttaaaaaacgctga
ccttggaggttcagtggggggggttcctctccatatattccaatgaaagaacatttgtttcgagcattccagaaatgccacgagttcctcagaagcacgg
tacaattccactcctacatcttcagccttattccttaccacatgccttttgtaggaggtacttttttttttctccggttgcgaatggaaccaaccaatct
tcctattgttcacctccagccagtgcactttctaaactgcaacaagtgtcaagaagttctgcatcgcgcaacgttggcaacgtcatgggaacttaagcag
aaacgatgccaactcttttgatattcctcaattttatgagattttattttcctgacctcatcttaatatcaacacctgacctgtgtcttaaacctcctca
aacactccagactacctttaaagacactttgtccgcttttattttggctttgcctgcactgtctgagcttaggtggtcataccgtcttttggtggaaaat
attcacggacaacacctctttccttccaactcgtcgtagctagattaggctttctgcttgctccgaagaagcctatagctcttcattttccagtgcaaga
ggtgctgatgcttgaacggaatcatgaagggagacctcattataagctacatgacatctcggcttcctgaatgtctattaggtacgaggtggattcacag
aagtcttaattaagcgagaaccttaacgnactgtgctcgttgtgcaaactgttttttatcaggaattttgatggtcagctaaacgtacgatcaatatgac
tgctgacctgcaatgtgtttact
3' EST: CX983332 [+]gcgagaggcttagaacataattaaaaaaatacaaactgaaaacatagcaaaatataggatatttttttttttatagcaactgacaactaccagccacttt
tactactgacctggtatatattttgtaactttttttttcttaaaaagacatgtaaatatataattcatatttatacattttctagctatatacaaaagga
aagcattattggattcccccgcccgcccccatttttttttttttaggataaaaatctgactatatgtcgtgttttctggacaaccggaccttaggagtga
gataaagcctctagttccagggactttatatggggctttcagcgaaacacaggccttcagtcaaacttctagtaaacacattgcaggtcagcagtcatat
tgatcgtaccgttagctgaccatcaaaattcctgaataaaaacagtttgcacaacgagcacagtccgttaaggttctcgcttaattaagacttctgtgaa
tccacctcgtacctaatagacattcaggaagccgagatgtcatgtagcttataatgaggtctcccttcatgattccgttcaagcatcagcacctcttgca
ctggaaaatgaagagctataggcttcttcggagcaagcagaaagcctaatctagctacgacgagttggaaggaaagaggtgttgtccgtgaatattttcc
accaaaagacggtatgaccacctaagctcagacagtgcaggcaaagccaaaataaaagcggacaaagtgtctttaaaggtagtctggagtgtttgaggag
gtttaagacacaggtcaggtgttgatatta
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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