XB-CLONE-3538621
Clone Name: IMAGE:7719831
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Gene:
cnot11
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX983343 [+]
aaggcgcgggctgtctcggctgccacagaaggcagcggcgttacacttacacgctgtccgcattcccgccaacatgagcctctgtgggaaagaactgtcc
aacctgctgggcatcatgacggaggaggcctgcagtaccacgttcgagggcctctcagccgccttccatcactgcttctccaaagcagaccatttccgtg
tgggctccgtgctagtcatgctcctgcagcagcccgacctactgcccagtgtcccgcagcgcctcaccgccctctacctgctgtgggagatgtaccgcac
cgagccgctggctgcaaatcctttcgctgctgtcttcgctcacctgcttaacccacccccttctgctgtagaggaggtagagagacccctgtcaggattt
ctgcctccaattactccaccagaaaaatttttcctctcacagttgatgttggcaccaccaagggagctatttaaaaagacacccaggcagattgctgtga
tggatgtgaccagtttggctcaaccagtggacattagtggactacagttagctcttgctgagcgccagtctgagctcccaacacagagcaaagccagctt
tcccagtattcttagtgacccagacccagattcttccaattccggctttgacagttctgttgcatcgcanataatagaagcattagtcagcggcccanaa
ccaccaattgaaagccactttcgcccagaatttatccgtcccccacctcctcttcatgtttgtgaggatgagttagcctggctcaatcttatagaaccag
atcatgccat
3' EST: CX983342 [+]ggcatcagaagagtacattttattccaacacctattagtcatagaaatgtagcccgaaccagccaacaaacagtcacagaatatttacaaatttaaatat
ttaggaaaaagtatttctggaattgccctaaagtgtacaggtgaaaaactatttactgggcataaaatgcttttaaactgtaggaatacactatgccctg
agcacagtacatcaaaatgcttaaaacgaaatattaaaaaaaaaaaaaagttaatttacaagactccatctacagatagcagtttctttacaaaggcaat
gagaaaaatcttattttgaaggctttgcctctgaagggttttctccagtgtccaaagtttttaacaacctgaacaagccggctgcttcccggatcctgct
gaactctatacagaaagcttgcacctctataaacaagtcctggacgttgattatcttgtttctaatcaaggactggaggaaaacacaaactaatcgcacc
aaacgattctgcatatacttgtccttaatctgctcacatgtggatatacaatttgaaatgtaaaggtgaatgaactcaggaggaagatccactgctgttg
taagtctatttacaacttccattgaatgtaaagacatatccatgttgacaagcacagagaaatattctgttatttgacttgattgcattaacttgagcag
catttcaatagcaaccaacggattattttctaccaaatctgggagcttggcaggagtgagggcaatgtggtaaactaacttagggtctttttctaattct
c
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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