XB-CLONE-3538578
Clone Name: IMAGE:7720169
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Gene:
enpp1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX983373 [+]
caaaacgctttcactttgccaagaatgacagaattgaacttctcaatttttacactgaccccaaatggcaagttgccaagacaagaagtgatgccaaaac
ttgtgttggtggattccatgggtctgataacagatttgaaagtatgcaggtaagctttaaatggtaaatgtgtttaatttgctggtatgtataatgttcc
ttaagggggtgcgaaatttctatttacagtaaaaacatagtcattgaaataaagaaaaaatgtttaaattgctcagtgaatttctgtcttaatttgagtg
tttgaggcccctgtttttaccaggaatcatgctgttttataacattcacattctgtagcagagtacttgtctgtctattttgtatgtgcaaattttttat
ttttcttatatgcagaaaatgtatttttggtgaatctcagctttgagttaaccaagagatcaaattagtttatagacctaaaaccgagtggaaagaacac
tattgctgtataaaggtagcattattattgatacaataatcacttgacgacttaagtcttgatgtccttatttgtaattgttctgtttatttccaggcac
tttttattggtttcgggccaggattcaagtttaagacagaagttgaactatttgaaaacatagaaatctataatttaatgtgtggtaagatttcactctt
atacaatttgccagatgctttattatcacaggcattctcaagtagaaatcttttcttgttcttattacagacttgttagaaattgtgcctgcaaacaata
atggaacccatggcagtctaaatcatttactaagagaacctttttatgtgccatccttgc
3' EST: CX983372 [+]ttaaagagaaagactacattgaggggcatatttatgagtgtgtaagccaacatcaccactgatgttgcccatagcaaccaattagctctcttcatgtcct
agcacagtatagctttctcataggagtattaaaaaagtaactgtcaagtggggggcgactaaagtgttaggacatgttgtggggtggccatacttctaaa
tatcattttcttatatacctcctgaggactgctgcagcagctgttatatataactcttttgaagctgcaatgctttggtaacctttggtgccttcaaaaa
aaaaatcccacaaatgctgctttttacatttttgcttgaatattaagaaggatattgggattaactgctgctgcgctaatttgcacaagtgcagaaagac
acagcaaccagtgagcacttagcatttatcagtctaatgcagtaataacaaaaacacatttctgattggttgctatgagaaactacagttgcaaatctgc
tcagtgtttgtaaatatgccgttcatgtagatgaattatttaacaaattattattgtcttgctggttctgggggaaagcagggggttaccagagaaccca
agtcacctttcttcttttatcctgttacaaacggtttaagaaaggcgtgccctatcatcttgcaataacccgatatgacatttggaattaacatgttgta
tttatctaggaagaaaaataactggtccaaaattattggtctcagctaacccaccctacgttgctcgagcttttacaagttcaaataattattcctcaca
tactgggaggc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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