XB-CLONE-3538468
Clone Name: IMAGE:7720179
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Gene:
gpn1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX983450 [+]
gactgtccgcatcccgcaacggtggccggggccgaggggagcggggctctgtgcctcatagtcctgggaatggccgggtccggtaaaactaccttcgtac
agagactcgctgcctatctccatgggaagaattcccctccgtatgtgatcaacttggatcctgccgtgcatgagatcccatttcctgccaatatagatat
cagggacacggtcaactacaaggaagtcatgaagcaatacaccctggggccaaatggcggaattgtcacgtccctcaatctgtttgctacaaggtttgac
caggtggtgaagttcatagagaagaggcagcagaactgtcggtacgttctgattgacaccccggggcagattgaagttttcacctggtcggcctccgggg
caataattactgaagctctggcttcctccttcccctccgtggtggtttatgtgatggacacgtcccgaagtaccaaccctgtcacctttatgtctaacat
gctgtatgcctgcagcatcatgtataaaaccaaactgccatttattgttgtcatgaacaagacagacataatcgatcacagcttcgcagtggaatggatg
caggactttgagacgttccaggatgctctgaaccaagagacgtcgtacgtcagtaacctgacccgatccatgagcctggtactggatgagttctacagcg
ccctgcgggtggtgggagtctctgcagta
3' EST: CX983449 [+]tggtaatattctttaataagtcacaacaaaatatagacagtctgttggttacgtattcattttggggtgttttcctttaatttacagcaaaggggctggg
tatgaacaattggggtacatgggaaaggaggagccaggagatagccccgcctctagacacactgatccaatctagttgggtagaacaggttcatttacac
aatataaagggaacagcagttacaacctgttgggggttaatatcattggcccaaataacggggtgcggatataaccagggggcacttacactggcgcaca
gaggggcacaaacacaggaatagtgggggggaaatctcataaacacagccttgtgcaaagggaatcagtgtgtgtggctgtagctggacagtagggagcc
aatcacaaagaagctggcaataaccaatccaaacacatctattagaaaaggatgtaaatctgaaacacactgtagccctgccccaccctcagcccatttg
gtaccagaaccactcggcctggatcgctaatggacttctctggtctctggtaccgagagatctgttattccttttcccagtaccaggggctgaatgggtt
tgttggagtaggacagtgacttctgattggctgaacatactttatactgatatacacaacacacaaggccagggtgtgactcactattgtttgtgcccaa
gtgccccagtataagtgcccctgtgctgggtaaaacacaaataccccagcctgggggagtccattgtcctctattcaggaggggttcagtaccaggagcg
aggct
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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