XB-CLONE-3538453
Clone Name: IMAGE:7720133
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Gene:
mtor
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX983461 [+]
ctgacatcagatattgtgttctggcatcattagatgagcgttttgatactcacttggctcaggcagaaaacttgcaggctctttttgttgccttgaatga
cgaagttttcgagatacgggagctggcaatatgcacgattggtcgtctgagcagtatgaaccctgcatttgtcatgccatttttacgcaagatgcttatt
cagattttgactgaacttgaacatagtggagttggcagaaataaggaacaaagtgcacgtatgttgggacacctggtatcaaatgccccaaggctaatac
gcccctatatggaacctatccttaaggcactgattcttaaattgcgtgacccagatcccaaccctggagtcgtgactaatgtattagcgactattggaga
actggcccaggtcagtggtttggagatgagaaagtggatggacgaactattccccataattatggacatgttgcaggattcctctttacttgcaaaaaga
caggtagctctgtggacattaggacaacttgtagcaagtacaggttatgttgtggaaccttacagaaagtatccatctctgctggaggtgttgcttaatt
ttcttaagactgaacaaaatcagggcatacgccgtgaggcaatcagggtactggggcttttaggtgcactggatccatataagcacaaggtgaatatagg
catgattgaccaatcaagagatgcctctgctctaagcctttctgaatctaaaggcaaccaagattctgcggactacagcactagtgaaatgcttgtaaat
atgggaaacctgcctctgg
3' EST: CX983460 [+]taaagagatggaacggaaaaagccttgcacagcagggacagtatacaataaaagggtgtgagatatatcctctgaaggttttttctgcacaggagaagac
acagggctgttgtcattgctctcgccttcactctcactttcactgccttcgggaggcgcactggcagtactgggatgacgcacctttttttcatcacggg
gatgattctggtgtttgaaatgcagaactgcctcaaagttcatcacagcccaggcatgccaggccttgtaccagttgtggtcatgttcagtggctgcact
gtaatactccagaacacatggaatagtacattcattaatgccttgaagattcaattgccattcaccaagtttcaaaaaacacctggccatgagtttctgc
agctcttgtttgtgctgagggtcatctgccacatgctgagcctgaagttgcatattgttcacaaacttctgcatatgctggaatgcctcaatctttcgag
tgctcttccacatgtacttcatgtaggcatatgtgacctgtggatgtgcagtaggtaagggctggtcgctttcaagagctgggtcaactcctaagagcat
aaccaatgtcttatgggacaaagccaaacgtccactcttgctacataggcttgcatatttcagccaagttctcatatcctcatgcgggctcaccacaaga
gactgaaccattaaaatcttctgccagtcttcgacaatacgctgacatccctgcagcctatcccacaggtctggcgtataatttcacggcgctcggg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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