XB-CLONE-3538390
Clone Name: IMAGE:7719853
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Gene:
eno1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX983508 [+]
ttcaccttcaaaagagtttattgcctcttcgtgtctagagtgtgacaaaccattatctagtctgaaactgcaataggatgagaacacaagactacaaagt
tgacatgaccaggaccactgccacaatagaaaagacaaggactacatgcaggcaccagtgtgacactgaatttggaataatcctaagcagcaaagctttt
aaactggaaagacagcaaggttatgctgggagaggctgtaacatctaacattgctggaagtgcctgaaattaaatgagggctcagcagtgacatgtaaga
cctcctgtagtctctcctaggtgcacagctactacatcagttgggtgagagcctaaggatgcctccatttagttgaaaacaggttttctgaaatttcttc
cagcaaagcgggccttgctgccaagctcttcttcaatcctgagcaattggttgtattttgcaagtcgctcagatctgcatggagcacctgtcttgatctg
tccagtgcggagaccaaccaccaggtcagcaatgaaagtatcttctgtctccccagagcgatgactgaccatcacaccccagccatttgactgagccagc
ttgcacgcctgcagggattctgtcactgtgccgatctggttgaccttcagaagcagacagttacaagacttctcatccacagccttggcaatacgtttag
ggttggtcactgtcaagtcatcaccaactacctggatattcgaagatgcagtaaactttttccaggcttcccagtcatcctgatcaaagggatcttcaat
ggacaccactggataattcttgacaaagctcatgtacaactcagccagctcgtca
3' EST: CX983507 [+]aaatttttgactctcgtggtaacccaacagtggaggttgacctgtacacatgcaaaggtctcttccgtgctgctgttccaagcggagcctccactggcat
ttatgaagcactagaactccgggacaatgataagactcgctacatgggaaagggtgtggctaatgcagttgagcacatcaataaaaccattgcacctgca
ctgataggcaagaatctaagtgttgtggagcaggaaaagatcgacaacctcatgattgaaatggatgggactgagaataaatccaaatttggtgctaatg
cactcttgggagtgtccctagcagtgtgtaaagctggagctgcagagaaaggagtcccactgtaccgtcatatcgctgacctggctgggaacccagaggt
catccttcctgtaccggcatttaatgtgataaacgggggttctcatgctggaaacaagctggctatgcaagaattcatgatcttgcctgtgggtgcagac
acctttaaggaggccatgcgcataggagcagaggtgtatcataacctaaagaatgtcatcaaggctaaatatggacaagatgccactaatgttggtgatg
aaggaggctttgctcccaatatcttggagaataaggaagccctggagcttctgaaaactgccatcaacaaggctggctacccagataaagttgtaattgg
aatggatgttgcagcatctgaattctaccgtgacgggaaatatgacttggacttcaagtcccctgatgaccctaaaagacacatctcctctgacgagctg
gctgagttgtacatgagctttgtcaagaattatccagtggtgtccattgaagatccctttgatcaggatgact
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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