XB-CLONE-3538360
Clone Name: IMAGE:7720095
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Gene:
cela3a
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX983528 [+]
atgactccgcccagattcccggccaggattcccgggaaggatctactggaatccacttacggaattctcactgatggtttgctggatccaagagttaaag
gccgacacgcgagtgaacacactgggcttcttgagggtgttgcacccgtagccagacccaaagctggtcaccccatgtacgtaccaccggccatcggccc
cctggcagttcaggggtcccccagagtcaccattgcagacagagcgaatgtcccctccggcgcacaccatgctgcgtttcactgtggcgccccaccagtc
tctctgggtgcagtgatcataatccaccaccgggagcagcgcctgctgcaggatatcgggatagggcccaccagtgtaaagtcgcccccagccactggca
tagcaggagaaattgttggggagcagttccccggcgggggggaggcagctcagttgaaccttatcacttatctgcacagggcggggcaatcggataaggg
caatgtcattcccacaagggatacaattattgttccatgattggtgcacaaacatgtcctcagatggaataaggaagatctcctctgctccttcctcatt
ggctaagtcataatctccaaccacggcccgattggtcctggagaagttgatacagtgggcggcagtcagaatccatctgtcagcgatgagggttccccca
caattgtgtataaataccccatttatcangacctgcagagagacctgccaaggccaactgtagggtttggcgctttcaccattcaccactcgggcagatg
gagcgta
3' EST: CX983527 [+]tacgctccatctgcccgagtggtgaatggtgaaagcgccaaaccctacagttggccttggcaggtctctctgcaggtcctgataaatggggtatttatac
acaattgtgggggaaccctcatcgctgacagatggattctgactgccgcccactgtatcaacttctccaggaccaatcgggccgtggttggagattatga
cttagccaatgaggaaggagcagaggagatcttccttattccatctgaggacatgtttgtgcaccaatcatggaacaataattgtatcccttgtgggaat
gacattgcccttatccgattgccccgccctgtgcagataagtgataaggttcaactgagctgcctcccccccgccggggaactgctccccaacaatttct
cctgctatgccagtggctgggggcgactttacactggtgggccctatcccgatatcctgcagcaggcgctgctcccggtggtggattatgatcactgcac
ccagagagactggtggggcgccacagtgaaacgcagcatggtgtgcgccggaggggacattcgctctgtctgcaatggtgactctgggggacccctgaac
tgccagggggccgatggccggtggtacgtacatggggtgaccagctttgggtctggctacgggtgcaacaccctcaagaagcccagtgtgttcactcgcg
tgtcggcctttaactcttggatccagcaaaccatcagtgagaattccgtaagtggattccagtagatccttcccgggaatcctggccgggaatctgggcg
gagtcat
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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