XB-CLONE-3538260
Clone Name: IMAGE:7720153
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Gene:
eif5a
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX983600 [+]
ggatccatcgattcgtgatgacaaaggcctcgagtgaaaatctgtagcagtaacactcatttgtgccacaaatgcctgctgtgtagtatcacccaacata
cagattgcctgtctagactgtaggcaggctttgacaccattttataagtttacaatatgcaagccaatgcattggcataggagagtacagaggtaataca
aattagttgcacatggccaccaggactaactttttcttttaatatggaggggctgggaaagtggcaaatcaactggcattttaaaagaaaaaaaaaaaaa
aaaaattcactcagtagtccaaagatcttccacactgagatttgaaccatagccaaacatgcataagatttaaacttgctccttaagttaatatccaata
attctaggtcttcttattttctttacatataagtaaaggtataccaaggtgtttttgtttttaataggacagcccagttgaactacctttgaattctagg
tgctatttacagattaaaccaaaggcctaattaaaggacatctgtttaatcatgatgcatacagtttagatgctttatatgtttagttggaccctcaaaa
taagcaccaatagaaatatgaaaggtaacaaccaaagcattaacaaaatttaaaactgcagtgaaattgaaggttgccagttgacttgaacaaccaaatt
tagaaaccagcagtaattgcaaaacaagtcaaaaggaaatgaggtcatttgtgaaggccatagctttggtgacaatcagaaccaaatttaagatctgtgg
ttatcttgacctgagcagattgc
3' EST: CX983599 [+]gggcgagagcttaaacaaacttttctttttgacaaaatgcctaaaaaataagaggcttaacgagaaaatgagaagttgaagagggcggatgtaagaccaa
accttcgttcccaggggaacatgactgacagtctctgttaggttaaagtgttttggttacaaagggtaacttgatgactttcactgattcccattttgca
cattttttaacagacaccacatactgtaaatcttatgcctcggacttagaaatggttttaacagactgaaatgtttgaagtcacattgcattgatagcac
atttgagcgcaggctacagacaatccctctcctgtggctattgctttaaaatattaaaccaaccatgggagagatgagtaaccagacagcaggcaccaga
agcagagcagcacttgttttggttgtgggagcagcattacgctggggagggcagtccttgtagggaggcaaacaagctgcatatgccatgacatgggcta
catatgtctgctcctggactccatgaaatagatgtgacataggcccctttgagaatatggccacatcttccccgccatgggtttcagaatccaaaggcac
tgctgcctgctgcaaatagccaacttcctcacttatattgctatccacattaggacggccttggccagttaggttgaacccaggcccattaccataaaga
atggatgtgtaagacttgttgtcaaaagctttctttggagccagaccaaatatgctgcttcctctataagtataaccccccaaggtgaagacatgggagt
ggtcagcagt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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