XB-CLONE-3538223
Clone Name: IMAGE:7720014
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Gene:
sbno2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX983626 [+]
ccgaggctttgtgggtcctcaagtgggtcctgcaaaaaccttatggatgtattatgtgctttgcaggtatacaataataacaggattcacctgtaagaac
taaacgggaagtttagctgctagagctgttccacttgggggacactcagaataccccacaatgacaaacaaagactcattctgacaataactctatgtaa
caacacatccgttactccacactccttactaaatgacacacctcagctcgggaccatgtttccgatgttcagcccatttgtgttgaaaacagtctgtgga
gctctgagtgcccatgtcctcttcagcggaaggctgataaggatgtgagagccaccatcttccctagtgcttgttttcggttcattcctcccacaccatt
ccctggaaacatgtttctgggggtgactcctcacagcgctcagcactttatcccctcctccacagagcattatcaaatcattggcccgcagggtcgtgtg
gagcaatagtttgtgagttggttgtaacatttgtggaggtgggtgggagagatcagattggatttttattttgtatcgttatttatcctatgctaatctt
tgcactaattgccttcctcctgtcctgccctgcccatcagcgtgtggtagggaagggcctgggtgtctgctcagggtatgtgtgcgtgtgtggatatgta
tttgtgtgtgtgtgtatatatatatatatatgtgtgtgtatatacatatatgtgtatgtatatatatatatatatatatttgtgtgtatgtgtgtgtctg
cggtttgctgaaactgtagcagccactctttcttttaacttgatgcaaaagaaatata
3' EST: CX983625 [+]ggaaagctgagtagtggcatataaagacattatgcctgcatttaaggttgggtcagtaactgaaatgaatggaattcttggctgaaaatcaaaattagtg
aataaaggattcatctatacagattccctcctgtgggacagaaaaaaaagaatgttctgaaaagttgtttccagtaaataactctgcttgcaatgggctg
caatgttacaaagcataaataaataataaatgactacgccagactcttttccctgcttaaaatctactgctgatgcctgatgccacaggggataaggttt
atgtcttaaaaggaatagaaggtaaaattcatgtcaggaatataaaacaaaggagatgggatcctcactcagcacagcactgtgctggggcaacatacac
agaaagcatggcattgaaatttgctcctgaaaatatttctagagataaagtgattgaacaaataatttacaactgttatttagaactgtgacagtttggt
tttagatttaaatggatgcttaaaatttatgacaaatcaactgtcacactcctgtaatgtttactctatgtgtactgtgtgctgccgagtgctccatgtc
tgcagcttccacacgtactggattttaaggaaatatattttctttggcatcaagttaaaagaaagagtggctgctacagtttcagcaaaccgcagacaca
cacatacacacaaatatatatatatatatatatacatacacatatatgtatatacacacacatatatatatatatatacacacacacacaaatacatatc
cacacacgcacacataccctgagcagacacccaggcccttccctaccacacgctgatgggcagggcaggacaggaggaaggcaattagtgcaaagattag
cat
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: