XB-CLONE-3538208
Clone Name: IMAGE:7719872
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Gene:
fdft1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX983636 [+]
acaagaacttttatttaataaaaatgaaagcacattgtataacaatgtcacaatacaggttatttccaacactcctacgtgtacaggagttgaactgcta
ctcccagcgcattattatctacccgtttaaccagtcgggcagaagcctgagccgattgggctgaatctttccactgagatgcagggaaccaatcatggtg
agttgtgccgtcaattagaacatgcaacacttgaacagcgacatcacatgtgggcggagtctgtttatagacggctatgactaatagggcacattggtaa
gtacagcacagagtcactatggtaggtctgtcagcacagggaaatgcatggtgcaggcttccctgtgtgtgcggctccataggaaactatgtgcctgatt
ttctcagctcccagaaacatagctgcaaccagctccactactggaacagatagttcattacctgcccagaacacgggggaaaccgtgcgctctgtaatct
tgcccctagcgtgacccaccgatcttagtagcagattcccagtatctcaggaaacaacaatttgctaaaataaactaaaattccaagaatatgctgaaaa
tcttcctttaaaactgctggtttatggtactacctattaaaataacccgtataatatttatatctgcacatcagcccatttcccattggcatacacattt
ccagttagtccacaatgaccagcctgcctctctcctgttaaactacaatcccatagttgagtaaacaattctggtgcaactgttgaatgctacatttcag
cctgcatgtgttgctattgttcacacagattattggattactgtagcacagtgtggctaaatacagaacaaggcagacgc
3' EST: CX983635 [+]tggctagtgactgctaacagcttagagcagggatcctcaaactgcggcccacgggccagatccggccccccaaggtaatttacctggcccctgctgcgtc
ctcacccctggctactgtctgtctgcagaccctgcgggagagcagggagccggaggatgcagtggggaacagggagtgggaggacgcagcaaggagcagg
gagcgggtcgtagtttgaggaaccagggggcatagtttgaggactacagtccaacccccccaacagtctgagggaccatgaactggtcccatgtttaaaa
agtttgaggacccctggcttagagagctgaaagcaggaagtagtgttctggctattatgttagacatctgcctttatagattacatttttgcctaactat
tttacaaatattttttattttgctcagtctgtctattttacccagtttcatgtttacactgacctgttcctttagggcgcaccatggtctgtgtaattat
tctgctcccctctctaggtgatggccatcgccaccttggccgcatgttacaataaccagcaggtgtttaagggggtggtaaagatccgcaaagggcaggc
agtgaccatgatgatggacgccaccaacatagaggcagtgagagccattatgtaccagtatgtagaggaggtgagtgctgtgccgccgctaaactcgcct
cccataggtggagaaaaggacacacccaccagcgttattactatatattataatctcccccttttaatctgcagatctatcagaaaatccccctgacgga
cccctcgtccggccgcacccaacacatcatcgtttcaattaggaatttgagtctgtccgacggctcgttagcg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: