XB-CLONE-3538202
Clone Name: IMAGE:7719968
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Gene:
slc6a8
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX983640 [+]
cattttttgtgcatttaacagatgatcacagtgcaaaagcatggagcttcctgtgttatggtaaagaggtatattcctaaaggaagacatagatcctcac
taggatcacatggtaatggcttcatattataagaaccaacaggctgcctgagacattgggcatgggtgcggaagagaaatatggatagccaacagttact
acattctaaggcaccttacaagctaatgctttccatgctggatggcagaactcatctaactgagcacttgactaaatctggtcctgtgtaattgcaggac
tcccacccttccaacatgaaagggaggaattttacctacacagtagtgcaacataatttcatggatagatatattattttggaggactaattctacacta
acccagaagtagacaagaagcctcatttttgtcttagagtccccatctgatgcttggggccccaaggacaaatggtttgtctaccagctgttcacaaagc
agctgtgactgagctgtgtaagtggcctaaaactatgaaaagggtttggccagcaaatgtattgcatatggcatatttggtaaagtccatttggatggga
aggaagctgcatgagtctaattaatttacaggagcttatgcatcatgagattctactagcaagcctctgaatcactaacctaaagcgccaatcgaagttc
tacactgatctgcaggtgttcctcacgcagaggagtaagttgtagcaaccaagctgaacttccacccatcctacagtatgcctgtcactgcctaccactg
acatgaatggaaaccacctggcaccactggaaaagccttg
3' EST: CX983639 [+]gcgtataaaatttctgaacagaagttatagggctcatttgcaaacctggaggggaagggtgcaagtgcaagaacactaagggaagcaagtcataggattc
acagagggacacagttataggattctaagctatctgtttaaggtgcttccctgaatcgctagtgattgttttaaagcctgagaaagggttaaagggacag
tggctatatatattggtctgttttggccatcacaagaagatttcacaccgctgactggctgttttttatggtaaaaaggaaaggccaagacaagcccatt
tgtaccaggaaacttacattaaaaaaagtgcctataaactatattactggctgactaaatacccaattttccagaaaatataattatataaataagaaaa
attataatttgacgtcaggtcccctttaaaatatctataattgacggaaaaggacatgtgcaagataactgaatttattgacatgactatttgtgtatac
atatatataacagctgcttttggtgttaccagcacagcctggaatttacaatatacagttataataaactttggctattgatttccaaagattaccccct
aatttaatgaatattaatctatttgagtgaaataagaaccagctcaggtcttccatgacagttagctgattggaggtttcctcagtgctcgtaggtaaca
ttcaccttacagagaccgctctccgcgctctgctcttaccctatgggactttagaagacattatgtattataaacactatgtattttattttctgtacat
tatttttgaggactgctgctttttctttgattctatgttgtaaatatgtttttaattttttgcttacttc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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