XB-CLONE-3538157
Clone Name: IMAGE:7720068
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Gene:
slx9
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX983672 [+]
cgggtcgggaccgccatagaggaagagccgccggtcccactcagtgaaccctctaatggcgcagatctgccgggggcacagggctgggagagtcaggggc
cagggaaggattggacatttttttcttctaatattttttcttcaactaaaatcaaccctgaaagtttaacacagaaactggaagtggacacaaaaagtgt
aatatctgctaaaaaagaagagaagaaacttgtgccaaaaaaggaaaaagctaagcaaagaagggaacgatggctgcaaaaaattgatgtcctggcccta
gcccaacaagccaaaaaagcccaggcaaaaaggaaagctactcctgtagttggggatatgaggccactcgttgatgcacttcctgaactgtcagaactga
ctgctgttcgcaaacctagagcacccagaaagcagcccaaatgcagtgtgaagaagaaaccagaaccaacagactacagcaaaatgaggcctgcccagaa
acgcaagattatagaggaggaagtgtcccgtgtgcaggaactgatcaggaatccttcttacaaagcaaatccactggcggcagtagggcagcaccttctg
aaacgtatgaaagatgagcaagaaactgaccccagatagccattgctatccagaaaatttacagctattgctacacaatgctagataatccagactgtga
ccacagaacaccagataaaaatcgcagcgactatggaacaccacaacagggagctgttaatcggtcacagctgcagatacattttgtatttctaaacagc
ttgctaaagtattttatttctgtatggaac
3' EST: CX983671 [+]tttttttatttagaatgatatttttattcaacttaagcataaaaccacatacatcatttgtttttccatgcttaactaaaatgtttctggtagttccatt
gtcactagcagtctagaagatcgttgcggcttcctttatagagttttttttatctccgttccatacagaaataaaatactttagcaagctgtttagaaat
acaaaatgtatctgcagctgtgaccgattaacagctccctgttgtggtgttccatagtcgctgcgatttttatctggtgttctgtggtcacagtctggat
tatctagcattgtgtagcaatagctgtaaattttctggatagcaatggctatctggggtcagtttcttgctcatctttcatacgtttcagaaggtgctgc
cctactgccgccagtggatttgctttgtaagaaggattcctgatcagttcctgcacacgggacacttcctcctctataatcttgcgtttctgggcaggcc
tcattttgctgtagtctgttggttctggtttcttcttcacactgcatttgggctgctttctgggtgctctaggtttgcgaacagcagtcagttctgacag
ttcaggaagtgcatcaacgagtggcctcatatccccaactacaggagtagctttcctttttgcctgggcttttttggcttgttgggctagggccaggaca
tcaattttttgcagccatcgttcccttctttgcttagctttttccttttttggcacaagtttcttctcttcttttttagcagatattacactttttgtgt
ccacttccagtttctgtngtaaactttcagggttgattttagtttgagaaaaaatattagaagaaaaaaatgtccaatcctttcctggcccctgactctc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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