XB-CLONE-3537924
Clone Name: IMAGE:7719946
|
Gene:
eno1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX983830 [+]
tgcacgtgaaatttttgactctcgtggtaacccaacagtggaggttgacctgtacacatgcaaaggtctcttccgtgctgctgttccaagcggagcctcc
actggcatttatgaagcactagaactccgggacaatgataagactcgctacatgggaaagggtgtggctaatgcagttgagcacatcaataaaaccattg
cacctgcactgataggcaagaatctaagtgttgtggagcaggaaaagatcgacaacctcatgattgaaatggatgggactgagaataaatccaaatttgg
tgctaatgcactcttgggagtgtccctagcagtgtgtaaagctggagctgcagagaaaggagtcccactgtaccgtcatatcgctgacctggctgggaac
ccagaggtcatccttcctgtaccggcatttaatgtgataaacgggggttctcatgctggaaacaagctggctatgcaagaattcatgatcttgcctgtgg
gtgcagacacctttaaggaggccatgcgcataggagcagaggtgtatcataacctaaagaatgtcatcaaggctaaatatggacaagatgccactaatgt
tggtgatgaaggaggctttgctcccaatatcttggagaataaggaagccctggagcttctgaaaactgccatcaacaaggctggctacccagataaagtt
gtaattggaatggatgttgcagcatctgaattctaccgtgacgggaaatatgacttggacttcaagtcccctgatgaccctaaaagacacatctcccctg
acgagctggctgagttgtacatgagctttgtcaagaatatccagtggtgtccattgagatccctttgatcaggatgactg
3' EST: CX983829 [+]caccttcaaaagagtttattgcctcttcgtgtctagagtgtgacaaaccattatctagtctgaaactgcaataggatgagaacacaagactacaaagttg
acatgaccaggaccactgccacaatagaaaagacaaggactacatgcaggcaccagtgtgacactgaatttggaataatcctaagcagcaaagcttttaa
actggaaagacagcaaggttatgctgggagaggctgtaacatctaacattgctggaagtgcctgaaattaaatgagggctcagcagtgacatgtaagacc
tcctgtagtctctcctaggtgcacagctactacatcagttgggtgagagcctaaggatgcctccatttagttgaaaacaggttttctgaaatttcttcca
gcaaagcgggccttgctgccaagctcttcttcaatcctgagcaattggttgtattttgcaagtcgctcagatctgcatggagcacctgtcttgatctgtc
cagtgcggagaccaaccaccaggtcagcaatgaaagtatcttctgtctccccagagcgatgactgaccatcacaccccagccatttgactgagccagctt
gcacgcctgcagggattctgtcactgtgccgatctggttgaccttcagaagcagacagttacaagacttctcatccacagccttggcaatacgtttaggg
ttggtcactgtcaagtcatcaccaactacctggatattcgaagatgcagtaaactttttccaggcttcccagtcatcctgatcnaagggatcttcaatgg
acaccactggataattcttgacaaagctcatgtacaactcagccagctcgtca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: