XB-CLONE-3537801
Clone Name: IMAGE:7720148
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Gene:
bag6
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX983916 [+]
ataaacagcaaagaggtgcttgtatgattgggcttcacttagggtgattagacccccttatttagacctaggtttagtagggtatgtgtgagaagtgcag
ggcaaacaactgtaaggctttgttgtatacatattacactaagaaaatgcaacgtctgtgtgcagcgtgtactgggataccatcaaaaaaaccactaggt
ctgtagggtatatacataactgaaggagagttctgagtgattattgtttaggggtcccctctgaatgccctttgtgtgttggggaatctctgggcactgt
aggactcattgtcttggaggatgttctggatatcctgttctctgtaccttccttgtgcttcactgttatccagctccctcctaacacagtctgtactgct
ctctggtttggctcctgtggctttcattgcacggctcacagcttcagagagggacaagtgtggcccttcaccttgcattgttttgcggcgctttgcaggc
atgccactcaggtaggcatcactcaagggtggctgctgtttcatcttccgctggttctgcatatcttgtctgattacaggaacccattctgggggcacag
atgctgcccattgctcagatccggtatcttcttgggttgggtgctcagaatctgtgcatatggtgggtactgaactttgaggggggaggaaaagcacctc
ctccactgtggtggcaggaactggagaggcagcaccattctgacaatcaacaggtgtggtttccataggttgatcctgagatgatgcttcaggagcctgc
ggaacatctcccactc
3' EST: CX983915 [+]tatagggcgagaggttttcactagtgttgttcagggtgtcctttcatccatgctggggtctttgagtgcagctgatcagagtggaacagaaagcattgca
gctttcattcagaggctcagtggctcccacaacattttccagcctgatgcagagggacctggtggtaggaaacttccctatggcattttccttttgcccc
tttctcgttttgtatatgtctttttttctttctctacaccccttcaattgtttcttccatttactccagcttttttctttttagaattctttctctaaaa
tttggcctttaacttgtttcctgcctcatttgttccaggattttttggtgacttactcaccttgatttgtcacaatttctccctggtcgatatggtaatg
ttactgcatggtcattcccagcctttgcagaatctgcagcctcaactgagatccttctttcttcaggagtatctacaccaggcagatcccacacctaata
acatccagatggcatcaagaaatcttactaatggcctggaagagtacataagagaaagctttgcctccgttactgtgcgtgatgatgttgatatcacaag
aacaaaccttgaatttctacaggatcagttcaacaggatcacaacacacatcctacattgtgcagattccacattcggacagcggcttcttgagatgtgt
aaccagtctctctttgaatggcttgctctcaacctgtattgcctccgaggggaccagagtgctctcacatctgtcattaatgagagaattcgaagactct
ctctangatgttcacctgtgcttgtctcgtgggtgacgtctgtcctgtcc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: