XB-CLONE-3537782
Clone Name: IMAGE:7720102
|
Gene:
enpp7
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX983930 [+]
attccgcgtttttaatggccatccgccagctggaggtctagtgtgagggatcagtggatgtcatttctgttgagtgctaactcttggtgcatgctggaac
attacattgatcatagagtaaagccaactgaaaagggaatattggcttaggatggaagaaagtccttacagcctctaagagttaggatatgtctttttag
gatgtcaacttttatctgtaatggtctccgtactcaaagatttctgggctcttttcctctttttgtgtctcttgacagaaacagtaatcattgtaataat
aaagctgagaagaagcacccccagaacagctgacagtccaattgttgcatgtagaatggatgctgggaaacatgctgggttgggatctttgtccaccagc
atgttccgtgtcacatctagggatccatcattgggctcaggtttgacacccagcagctcacacatcagggcatacacatgaacgctctcaaaaggctcca
caaccaaatcctgcttaaaggatggacccacagcacggaatatggtcttcatttccatatcatcattgtcaaagccatgctcccctttattaaactgtaa
ctttgcaaacccatgaacgacatatcctggatctccatatacaacaagcggtgtaatccgagcattgttggaatagtgcagcctggctggtacttcttct
tttttaaaaacattgagcttagggtgagcattctttagggcattatataccttttctaaattaccttccttaggtatcaaaagcccactagggtcataat
ctaccaaatgaaactgagggtcagaaagtgagaagtttggtaggttattga
3' EST: CX983929 [+]gcgagaggttccactagaggggcgcaacaaggctcctcacaaactcttgcttatctcctttgatggctttcgttgggattatgatcaagatgttgatacc
cctaatatgaacataatggccagagacggtgtgaaagccaagtacatgacccctgcttttatcactattaccagtccatgtcactttacactacttacag
gaaaatacatagaaaaccatggagttatccacaacatgtattttgatgtcaccactggtcagaagataccataccacggcacacagggcgtgtccatgtg
gtgggataatggtagccttcctatttggatcacagctcagagacagggccttaacactggttcgcttttctttccgggtgggaatgcaacatatcgtgat
gagaatgtgaatgtaaaaaaagtagagtcacgcacacacatatggggcaatgaaacagagtggagagaaaatgttgacactgtgatgcgttggttaacag
agcaggatttggattttgtagctttgtattttggtgaaccagatagcactgggcacaagtatgggccagactcagaagagcgcaagcagatggtcagcca
agtagaccgaaccgtcggatatataagaaagcgtgtgaaagatcatggtctagaggatagattaaacatcatcatcacggcagatcacggcatgcgaaca
gtacacaagggccctgatgagattatcctcaataacctaccaaacttctcactttctgaccttcagtttcatttggtagattatggacctagtgggcttt
tgatacctaangaaggtaatttagaaaaggtatataatgcccctaaagaatgct
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: