XB-CLONE-3537767
Clone Name: IMAGE:7719960
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Gene:
MGC75753
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX983940 [+]
ctagctctggcctgcttcccccccttcagcgaaacactcgggggtagaaagttgaggactctggctgccggagatcacaaaggaatcgcgtacgattctg
tcctgcctatacgatcgaagagagagacccgctgctgttgatgtttatatagctgtgaggattcaaccttatatgccacaatgatgactgagatctcaac
agctgctgcgtttatcacaagtttgctgaaaacaaccggggtcatagaggagaggcagctgcagcacttctgccaggtcctacaggaggccatgaagggt
aagataaggctagcctggcctctctttctctctcatacacagatggacccaatctgatcgcaccaattggaacaacgccaaggagcccactggcacaaag
accatcatgcagataaatggctacaaacatggccagcacaggcttattattatgtgatacaacaatgcagtctgctgctcaagggaatctacttgattcc
aaacaattctgattaagagttttagcttttcaaacattttgtaacattaaggacaatttgttcgaaattgaaccaactgtttaattgtaggatgtttatc
ttgtggcccctccacctgttgttttactacagcttccccctgtggcccttccatatgttgtggttttgggagttgtagtttaacaataatctaagggcaa
catgctggacgtatacctttgctaacactaaaaatacaactgatagaaagcaatggtagttttgtttagccccagtggctctgtgtgcctccctaacata
cagacaaacatctttgttttatcttccagggtggaacagtagactcatttaatcaggacattattattctgtctagttctaggct
3' EST: CX983939 [+]ctatagggcgagaggtggttataccaaaatataaattctctcttgactcatgatttcattttacaatgtacaatttcagagaccggagcgagaaaatgtc
ataaaagaaagacagaaatgacaataagagctttttgccccagatggtattctacattcaccttctgcaacaagacggtacatttttggcagtattacag
ttttaatgtttatggaatgaagatgaaacattaacgcaaatcaaggcagaagagggacacatacgtaattgtacaattcctgatttatttttcagctgtt
taactagaaactgtcattatgctgtaatttttggaggggctattccggccaattctaagtgcatccttgcagcttgcatggttttcaataggcttgcccc
ctgctgcggctccttcatacaatacacagatggacccatcctcaccaatgcgataggagacttcaaaagggtcaacccaaagagtcagctcgctggggag
aagctggaacagtctctggtggctgagcccaatgtgacctgctgcctgtcctatcaaggggtccattttgtggttaattctcaagcaacggtaccctgat
cctttgaaagggttctgcggaaaccagtggtgtttataatgatctgttggaaacaaagagaggtgggggttgcagggagggcaggggtttaggaggaata
gggggagggaccagataaagactcgtttagacacattcattcacataaccagtaattcaagtattgttcacagaanaaaaaacttcacataataagtaat
aaaactgtcatttttgcatgcccaaagagagtaaacagtgttttagttagaagtgcttgggtaacatttaaagaaaataggtacagtatttatacgtcag
t
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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