XB-CLONE-3537700
Clone Name: IMAGE:7720445
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Gene:
cyp2f1.2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX983984 [+]
ataaatatcaaattaatataggccgttctgcacataattcatcattacaaatacagtttacaggtaactatggttcttcgtgtagagtagaatggtttca
tggcttcaccataagatgtgggagtgtaattggaatagggaaggcgtatttgtcccaaaatgttctataaagcagagaaactgaggaataacaagggctt
tcttcactcaggctgaaaagtcttgatctgtgaaaaggggtggggttttgaagagtgcggacactgaccaggacttcaagaaatagctaaaattacaagt
aaataaaggttttggtttcttaagactgtttatagctttctatgtttgatttttgtaaatgagaagctccattttatggtgatgatgtgtagagtttgtc
tagctgcgaggcaatgcacagatctgatggtctaaaggtatggatgtgagtgcatctccacaggtcagttggacctcaactccaggagggggttggaatg
tgaagttctgcagaagttttgtgaagaacaagaagagttccatcttggccaacgtctctccaacacaacttcttttacctactgagaagggaaggaaggc
ctcatttttcacaaaatttccttttgagtcaaggaaatgttccgggtaaaattcttctggtttttggaaataatctttatcttgcataacagaaatcagc
agggggatcacttgggttccctttggaaggaaatagcctctgaatgtgacgtcctcagttatcgaa
3' EST: CX983983 [+]acttctgtctgttgtcatttgtattttcctatataaagtcttctatggcgggaaagaaacatccaagaacttccctcctgggcccaaaccattacccctc
attgggaatctgcacatgatgaatatgaagaaaccccatctaacatttatggagttcagtaagaagtacggcccagtattcagcattcagcttgggttaa
ataaggcgattgtgctttgtggcgcggatgcagtgaaagacgctcttattaaccatggcgatgagttttcggggaggcccaaaattccagtctttgacca
gatatctaaaggatatggtgttgtgtttgctgatggggagaactggaaagtgatgagaagattcgctctttctacgctaagagactttgggatggggagg
aaaactatagaagacacaatcgttgaagaaagtggctgtttggtggaaacgtttaagtcccatgaagcgaagccgtttgataacaccttgatattgaatg
cggctgtagccaacatcattgtgcacatactgctcaaccaccgctttgagtatcaggatccaacgctgatcaaacttataaaaagtgtcagtgagaatgt
caagattgctggaagtcctatcgtcatgctgtataacacatatccgtctattatgggatggattcctggaagtcacaaaactgttttcgaaaacttccaa
aaattatccaactttcttaaagagacatttaccaaacgtagggatcaactggatgtgaatgatcagagggatctcattgacgcattccttgtncagnnca
cagaggagaagtcttcatctaanaagtttttccatgatgaanatttgaaagttcttttaggcgatttatttgccgctggaatggagacgacttctaccac
tc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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