XB-CLONE-3537673
Clone Name: IMAGE:7720543
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Gene:
dram1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX984003 [+]
atttccctggattgttttacagcctgggctgcccaatattattattattattagttgatttaaaatattgtgtttgaactgtcccagctactgctttgtt
ccctttcattcgtatgctaaatctttcctacaacaataaaccaatacatatctttaaagacacattaactggtatgtacaaaggatctgtgtcactgccg
tcaaaatttgtttacaaaaaaatggattttgaaagaagtccattaggcaaatagcaaaagagaaacagagcttcttctggcaagcagaaagacagaagga
tctgtagatactccatttgtttacaaaataatgtccataacttgtgtcatccaagccacaaagagaataagctccttgttcttcaccaatcagagacctc
cataatactgtcctctcatgtaacatcagtaacaaagcccactgcaacatttgtgaactgtcaaaccatcaaggttaataggagacaaaaagcccttccc
aatgtaagaattgcagtgaaagccttatagaaacatgccaggtacatatgtagtattgtcttaaaggagaactataccccagaacaatgtaggtctctat
aaaaatatattgcgcaaaacagctcttattcatctaaataaaccattttcataaaactatactttttaggtagtatatgccattgagtaatcctatatag
aaaaactgccattttaagttgtgagtgccaccccctgggattgcacaattaacgatgcatacaaacaaaccaagggcacatatacatatgaggtcacatc
agccatgttgtactgcaaaaactgta
3' EST: CX984002 [+]agaggctttttttttaacaacaaaatacattttattttcattttctgttataaaatggtaagcaggctccaaatgtgttgtttccatccttataaatctg
ttcatactgttggattcttcacaattccagcaaacgaatgtcattcatgtgtacagcattgagaactagtgccctgtgcatgagggtaagtgaacctgag
aaaaacatggtgctgaattcttctaggtgtgcattgtgatggtccagccatctctgggagattataaaatgtgccattgcatggaaaactcacaatataa
aaatggcaaagttgtacttttgtggtgaactccacactccatgccattgttagatgccattattatagtcatggagcgacagtcgttcactgagtagtgt
ctaagcagctgccatgttatctctaacctagtaagcacctcagtagaaaagaagcaagaagcagtcttcccctacagcaatagcttctgcctttcctctc
aggctgtgctgcagttagaggcttaacatgactatgggacagtgaggataaaatacagcatggagcaatacagtacactatccctttaagtttgagattt
ctatacaatatttaattaggatgggaaagagcttgaagttatttgttatacataaaatatcccaacgctaccaagcaagtgaaatacagatgtgctgtag
atatatatatatatatattaatgagagtaatatattaaaaaaatactgttacaaatgtganaaaggacacagaaaaaatttaaaggcaatgtggcaacca
ataacattgcgcttagtagctcttactctggttgcagccagggctgacttttggtcttttgcgcctcacgtcacctcagcctctg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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