XB-CLONE-3537651
Clone Name: IMAGE:7720259
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Gene:
XB5762393
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX984017 [+]
caaatatttaccccacatcccttcctgcaggagaatcgggatctggttggactatgaggctggactcctgtccttttatgagctgagtgagccaatcagg
cacttacacaccttcactgcctccttcactgagccccttcgtgctgcattcagggtacggagcaatggtgcctgggtgagaatcattagagagagagaaa
agggagctgaagtactgagagtagagggagagacagacagacagataagagacagtgaggatctgaccccaggcagagagaagttggacccagagacccg
gatgcaggaggaggctgctgtgtctcatataatccacaagggggagacagagagggaagagttggccgggcagatccctgacatggaggaatggtgcagc
atggttgatagattgggttccttactggcacagggatcagatggagctgcactgtgtggggccgagggggcagataatgagggcagagaggcagatggga
taatggcccctggattcgctgaaactgttctgggtataaggagactgatatatgggccggaggctacagacctggtactggatataaacacggcccatga
tcatgtatctgtatcaggggacaggaaatctgctttctactcactaacaaaccaacattacccacaatccccagagagattttggtttcatcctcaggct
ttaagcagcaggagtttcccctcagggcgacattactgggaagtggagggcagtgaatcagggagctggggggtaggggcggcctatcccagtatagaga
gg
3' EST: CX984016 [+]ggcgagaggcgaaaaattactcagtacttagctaatcagcctgtggggccatcagttggtagatataagtcggcccatgtatgggacaaattacagccgg
ataatgccagagagcatttgtagacaaaaaacccaacccatgtgctcagtgacaggtgaattacaacttttgccaatgtaatatattatggggctgaatc
agctgtttctccaacagtaaaaaattattggtgaagaaactgttcatgtgacattcagcttggcctccccaagctcataattcaccctccacctatgctt
gctgggtagcaccctaactaatgattctcacccaggcacaattaacgtagagcagagcatgaaggggctcagtgaaggaggcagtgaaggtgtgtaagcg
cctgattggctcactcagctcataaaaggacaggcgtccggcctcatagtccaaccagatcctgattctcctgcaggaagggacgtggggtaaatctgtc
catttactgtcatgtctcactgaatatctgttattattccatctgtacaaaccccaggacttgttatcatacccaataccagactgccaccctctcctct
ctatactgggataggccgcccctaccccccagctccctgattcactgccctccacttcccagtaatgtcgccctgaggggaaactcctgctgcttaaagc
ctgaggatgaaaccaaaatctctctggggattgtgggtaatgttggtttgttagtgagtagaaagcagatttcctgtcccctgatacagatacatgatca
tgggccgtgtttatatccagtaccaggtctgtag
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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