XB-CLONE-3537578
Clone Name: IMAGE:7720457
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Gene:
med7
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX984065 [+]
aggaacggagctgtttgaaggttctcctactcacttcataatgggggagcctcagcaggtcagcgctttgcctatacctcccatgcagtacattaaggag
tacacagatgaaaacatccgcaagggcctggcccccaagcctcccctccccataaaggacagctacatgatgtttggcaaccagtttcagtgtgatgacc
ttattatccgccctctggaaactcagggcatcgagaggctccaccccatgcagttcgaccataagaaggagctgaggaagctccttatgtccatcctggt
caactttctggatatgttggacatcctgatccgaagcccaggcagcatccgaagggaggagaaactggaggacctaaagttgttatttgttcacatgcac
caccttattaatgagtacaggccccaccaggcacgtgagaccctgcgggtgatgatggaggtgcagaaacgtcagcggctggaaacggcagagaggttcc
agaaacatctagagcgagtggtggagatgatccagaactgtctggcatcgctacccaatgacctccctctttctgatggcgcagtcagtgtaaaaactga
accaatggatgttcgggaaccctgtacggaccatcatgcggggccccaggaggcagcagcagcttctctgaaagagaccacaattgataaggatgcagct
atgtgtgttatcatcgatgaaatgacctagtccaataagcagttctaatcaggactcttgaactttagaagtcacaaggattcattgaaatgcaatagca
tggctctatgtgttggttgtattcatcatgggactt
3' EST: CX984064 [+]tttttttttaacttttaataaactttgatatctcagaacatgtgaataactgtacaaacataacttgcaatgagtagattccatacatagtatatcagtt
agataacattcaaaagtctgaccccagtcctttgtctgtttaatcacaaataagcagttaccacccaggtgttgcagaactacaaccttccattcctcat
acacatgaactggcagggaaaacactgaaaaatattagccttcttgcatactgccttttcaaaagcatggcagaaaagcacaataaacagaaatgtacat
gagcaatagctgtgactgagttcccttgggacaaacattttccttgccaagtcccatgatgaatacaaccaacacatagagccatgctattgcatttcaa
tgaatccttgtgacttctaaagttcaagagtcctgattagaactgcttattggactaggtcatttcatcgatgataacacacatagctgcatccttatca
attgtggtctctttcagagaagctgctgctgcctcctggggccccgcatgatggtccgtacagggttcccgaacatccattggttcagtttttacactga
ctgcgccatcagaaagagggaggtcattgggtagcgatgccagacagttctggatcatctccaccactcgctctagatgtttctggaacctctctgccgt
ttccagccgctgacgtttctgcacctccatcatcacccgcagggtctcacgtgcctggtggggcctgtactcattaataaggtggtgcatgtgaacaaaa
tacaactttaggtcctccagtttctcctcccttcggatgctgcctgggcttcggatcangatgtccaacatatccagaaagttgaccaggatggacataa
ggagcttcctc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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