XB-CLONE-3537566
Clone Name: IMAGE:7720267
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Gene:
cela3a
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX984073 [+]
agaagagatgactctgcccagattcctggaaaggatctactggaatccacttacggaattctcactgatggtttgctggatccaagagttaaaggccgac
acgcgagtgaacacactgggcttcttgagggtgttgcacccgtagccagacccaaagctggtcaccccatgtacgtaccaccggccatcggccccctggc
agttgaggggtcccccagagtcaccattacagacagagcgaatgtcccctccggcgcacaccatgctgcgtttcactgtggcgccccaccagtctctctg
gctgcagtgatcataatccaccaccgggagcagcgcctgctgcaggatatcgggatagggccccccagtgtaaagtcgcccccagccactggcatagcag
gagaaattgttggggagcagttccccggcgggggggaggcagctcagttgaaccttatcacttatctgcacagggcgggacaatcggataagggcaatgt
cattcccacaagggatacaattattgttccatgattcgtgcacaaacatgtcctcagatggaataaggaagatctcctctgctccttcctcattggctaa
gtcataatctccaaccacggcccgattggtcctggagaagttgatacagtgggcggcagtcagaatccatctgtcggcgatgagggttcccccacaattg
tgtataaataccccatttatcaggacctgcagagagacctgccaaggccaactgtagggtttggcactttcaccattcaccactcgggcagatggagcgt
aagtgggaaccccacatccat
3' EST: CX984072 [+]atggatgtggggttcccacttacgctccatctgcccgagtggtgaatggtgaaagtgccaaaccctacagttggccttggcaggtctctctgcaggtcct
gataaatggggtatttatacacaattgtgggggaaccctcatcgccgacagatggattctgactgccgcccactgtatcaacttctccaggaccaatcgg
gccgtggttggagattatgacttagccaatgaggaaggagcagaggagatcttccttattccatctgaggacatgtttgtgcacgaatcatggaacaata
attgtatcccttgtgggaatgacattgcccttatccgattgtcccgccctgtgcagataagtgataaggttcaactgagctgcctcccccccgccgggga
actgctccccaacaatttctcctgctatgccagtggctgggggcgactttacactggggggccctatcccgatatcctgcagcaggcgctgctcccggtg
gtggattatgatcactgcagccagagagactggtggggcgccacagtgaaacgcagcatggtgtgcgccggaggggacattcgctctgtctgtaatggtg
actctgggggacccctcaactgccagggggccgatggccggtggtacgtacatggggtgaccagctttgggtctggctacgggtgcaacaccctcaagaa
gcccagtgtgttcactcgcgtgtcggcctttaactcttggatccagcaaaccatcagtgagaattccgtaagtggattccagtagatcctttccaggaat
ctgggcagagtcatctcttct
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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