XB-CLONE-3537551
Clone Name: IMAGE:7720507
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Gene:
smug1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX984083 [+]
cttgactccgacagaccttcctaaggcacaacgggaaaccttgctggagatatgtgatgaagccctttgtcaagctgttagggtacttggagtaaaactg
gttattggagttgggaggttctctgagcaacgtgcaagaaaggccctaacagccgaaggaattgatgttactgtaaaaggaataatgcatccttctccca
ggaacccacaagctaacaaaggctgggaaggagtggttagtgcccagttgcaggaactcggagtactgtctttactgactggatgataaggccaagtaac
cgaaaacgccagacattataaagatggcatttaagagtagactttggtcctggctctcctgccttcatttacatggacaataaaaggcttgtctctcttg
tacaattatgactttcatttcaggcagtaagctaggtgaatctgagaatgaattgtaatttatttatttaagtgatactgacactaaaaaactactcttc
aaaatattaatatacataaaaaattacctttaggtcatgttgattgtttttaactgatagttttgcttttgtaaataattgttacttgaaattcctaaac
atgacttttgccaacctgactgtcccttttcatgctgtcagttatagcttctagtgctaacagactcctgctgcacaaatatggcagcccccttgcaaag
gaacattgngatcagataggtaatataaaagcatcacttttatggcaaaattataaattgcatacaaaggcaaagttatgatagatgtaaaaaaggttta
ttttctggcatcagtatctcta
3' EST: CX984082 [+]atagggcgagaggcaaaaaaggtagtagtgtgttataaaaagaaagaaaacatggcagcacagaattccttctctccatatttgtttgttataaaagtgg
aaaattctgccttggctgagaaaagaattgtgggtaataactgcagctctctttaggcctatggtgcacaaggagtatacagtcagcattttttaggtag
taaggaaggaccccatgtcccataggccttaacatgaataaatacaaatatacagtggacatttactctattaacataaaaggacaatcttaaatatttt
actatctgaaggaacatagcatggacgctaacgctacatatacataggaaatatttaccctacgaccattttacaaaggggttgctgttcattaaaagtg
atctacaccacctcattactgttttgtcttacttggcccttattaaaaatgcaataaaatatgtacacactttacctataagagacattaaacattttat
attttccaagtcaaacactggagggctttttacacccttcttagaatagttaaaatcttggcccttttggcaagcagaatcacaaacctgaactggtaat
ttttcctcctgttaattaggagggaatagtttacataagcaatgagacaagatgggctactttatgaacgctataaagcagtctgagcatgccccttgcc
tcgctgcaccttccagacatacattagaacttgtaatgagccgtccgccttgtcctattactaccaagttctccactgtacgcaccaggttctaaagaag
aagaacatgctaacagcttagcatatggcagtagagacgaaagcacctgctgc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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