XB-CLONE-3537498
Clone Name: IMAGE:7720273
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Gene:
aqp3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX984119 [+]
cccagacgttgggagccttcctcggtgccggcatcatctacggcctgtattacgatgccatttggttctttggaaacgaccaactctttgtaatgggcga
aaatggcacggccggcatcttcaccacattcccatcggagcatctgacacttataaatgggttttttgatcagttcattggaaccgcagctctgattgtc
tgtgttctggccattgttgatccaaacaataacccgatccctcgtgggctggaagccttcaccgtggggtttgttgtcctcgtcattgggacttccatgg
gcttcaactctggttacgccgtcaaccccgccagggactttgggcctcggctcttcacatctctggccggatggggcactgaagtcttctgggccgggaa
ccagtggtggtgggtgcctatcgtctctcccttactgggagcctttactggagttttagtctatcagctgatgatcggatgccacattgagcctgcacaa
actcctcaatccaccgaacaagaaaatatcaaactggcagatgtcaaaaacaaggacagaatctgattccgctgccgcgaagggactgtatatattccag
ggacacagcaacgatataagaagggattccaaagccttattactctccaggagtcaccactcagctttgttgtctatgttagaaacttcttaggatgtca
aatttgtttccccttcttgtatagatatgtcttccgttaccatccagataggtctgtaataaagtcttccagtgacacaatatgcatattccaatgtcat
ggtggtggtgtatctccagtgtcatggtggttcatcttacagtgtcatggagctatgcatatttt
3' EST: CX984118 [+]gtataacaattgtgtatttaatatcacataaaacacattcagtacttaaaacaagcgacgtcgatcaaatgtatgtacaaagctttacatgtgtttgaag
tacaaacaggacctttcagcgggcgagatcctttccccccaatgcatcggccacttacaggtttcaacagatattttttatttattgatgttatcattga
gctgttggcacatctgacctggagcataaaggaacctacaatgataatgattccctatgtataacagtcattatttcccttttaatggtgattgggcagt
tccctgactccatgatggcgatgcagtgttgcattatataccatagagagaatgccttacttgtaaagggaatataaagcctattttattattcagtata
ttccccattaagcattaaaatggatgttctcaataggaatgattctcaaatacttcaggtttgtttcccaattacagttcaacttgtgagccaatcgtat
tttcctctacctggactggagtttggatagaattagagaccagcagaacctcaaatgcatagataaagcagttgcttgaaacacctttgttccatatcca
tttgccaccaaagcaagctatgtacaatgcaaggcaagagaagaaaaagacatggagggcaaatctttatggcagtggaagatacatcttcatggtcttg
taggacgtatctccacgacacagagtgatgtatttggaaagggatatcttcacaacaccggaaggatgaatctccatgacattggaaaatgaaacttcat
tggaagatgaacctcatgacactggaagagatgtctc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: