XB-CLONE-3537410
Clone Name: IMAGE:7720329
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Gene:
atp1a1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX984178 [+]
ctttatttgtaaaaatgcagatatataaaacatctctgaacgtgctcccattacaaaaagaaacaaaaaaaagaaacaaacgaaaaacaataccggagaa
ggcacaatgagaactctgtacaggacgcacacaggcgacactgcgccccttgccaaaatgcatctcttccatgggaacaggctgagtcagacgggtgcac
gcaggcgacgggtgggtgcaggcagaccgaggggcaggaagtgacgtgaaatgttcggacgacccgaaggctctaatagtaggtttctttctccacccag
ccgcctgggctgcgccggataatcagcttacggacttcatcgtatatgaagatgagcagcgagtatgggaaggcacagaaccaccaggtcggcttgagtg
ggtacatgcgcaatgccacatccatgccagggcagtaggacaggaaggcagccagtgcagtctcttcaaacagcccaaagataaggattttattcttcat
gccctgctggaacactgaattccttctggtcttgcagatgatcaaatcggcccactgcaccaccacgatactgacgaagaaagccgtatggcacgtgaac
tccacaatcttcctctgctcgtaggtccactgctgcccgtaactgtcctccacgtcgttattccagcggtcatcccagttcactctgatacccagcagtg
tccagggcagaaagccattctcagccaggatcacaaagtatgtgaagaacccccccagggcttggatcataccgatctgcccataggccatactgataag
cctttcattcaccagcttgtctgttttgggattacggggctgtctcttcatgatgtcactctcggcttgct
3' EST: CX984177 [+]cggcaaatgccgaagtgctggaatcaaggttatcatggtgacaggtgaccacccaatcacagccaaggccattgctaagggagtgggaatcatctctgaa
ggcaatgaaacagtggaggatattgctgcccgcctcaacattccagtcaaccaggtgaaccccaggtatgttggaaatgggttcctattggatggtaata
attctggcagtgtcggaggtgctgctatgacacgtgggaatgtgagtggctgagctctatggctccttgctgaatggccaatgggactgtaagttcagct
ttccgtatgtaacacatggctcttatcctgcagagatgccaaggcctgtgtgatccacgggtcggacctgaaggacatggcacaagaacagatcgatgac
atcctgaagcatcacacagagatcgtctttgccagaacatcccctcagcagaagctcatcattgtggagggttgtcagcgacagggtgccattgtggcag
tgacgggggatggtgtcaacgactcccctgctctgaagaaggcagatattggtatcgctatgggcatcgctggttccgatgtgtccaagcaggcagccga
catgatcctgttggatgataactttgcttctatcgttactggagtagaggaaggtcgtctgatctttgataacctgaagaaatccatcgcctacaccctg
accagtaacatcccagaaatcacacccttcctcatcttcatcgtcgccaacattcctctgcccttgggcacagtcaccatcctgtgtatcgatctgggca
cagacatggtccccgctatctccctggcctatgagcaagccgagagtgacatcatgaagagacagccccgtaatcccaaaacagacaagctggtgaatga
aaggcttatcagtatggcctatggg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: