XB-CLONE-3537347
Clone Name: IMAGE:7720335
|
Gene:
tra2a
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX984219 [+]
cagctaaccagccctgagggggtctaagttttagcatttgtgctgtttttatacacctacatgtaagttctgtaaatgactttggtcacagtgcttggta
attcataagcaagtacatagaaaccacctagattaggtagtttgggcgcaaagcaattcagagctttctaaaatcatacccctcttaatagcaatgcaac
atgggaaatttctcccactgttgatgccaatacctgtgcttttacaacagcctgcaggaggaaatttccaactgggtgattaagcagtgtgcaacattgc
tctaaatattcaccatcctagcaaagctctgtacaactcaatgtttagaatgtgggtaacaggataattttataagatattaaacattctcagtttcata
taaactcataggaagatgtagctaccatgcgtctgaaactgaaatatttgtttcaattcgtttctggtgcaggtactacagataagaggtcgggaaaagc
atttcttcaatcactaaactgttccaatgaataatgaatagcaagaagggagatgtataactttaggaaaagggataacttcatgaacctgcactgcgta
aatacaacacatcaatctggcaatttaagagccaacaggatttagaagagtcagaaaaaaaaaaaacattttaatatggtttattgtacaccgctttact
atgcacaaaaaaaaatagagtttcctttcaattaaataggctgcatgtttagcaaaagaaaagaagtcctttcaaagatcatagtggttgggaggggaaa
aaaacaaaaccctcgggaat
3' EST: CX984218 [+]atagggcgagaggcctgctatatagccttagtttagcttaaatccacaagttgggacaaagaaccgactggcaaggaaagctatatgtttaataccacaa
acccgtcataccttaaacatattcaagaatgaacaactttaattttttttagtaaaccataagtacccatgccatgtggtttcaacatcttaaccatctt
tacatttattgtagtttggtacatgctttcaaggggcactctgaactagaggattctagtcttgctactaagtacactgcaatagaaagaacattaccac
cttttagtataagtcacctgtcatgtccatccttgctcccacgtttaaattgtttttccaacaaagtctttgtccatttctctaggaatgcatagatacg
aggtgcaatttaaccctaagatgctatagctatgactgctcctgtgccagtgggcatatgcattatcgagtaaaagggttgtattgacaaaacaggcctc
atgggagcaacaaaggagtgggttttcactgggaatccttgcattgtaacagcaccaaaagcagtctgcagctgcttggatatattgcttcattgggtta
gttttggttgaaattctgcgttcctcaaaatgaccggtattatgcagtactatcttactctactcctgattagcaatggatccagtcaggaagatttaag
ctacaggcttaatgcttgtgcggngatggttacagctggttattttatttagggtaggttgcaaacttcttctaataggacacatttccatactttattt
acacacacctagattaaggtaccttgaattttttttcccttatttagtgtgcatattaagtgatgcagactctttccaggctgtatggtctggggcaggt
gtgac
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: