XB-CLONE-3537318
Clone Name: IMAGE:7720433
|
Gene:
cela3a
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX984238 [+]
caggagcctatggatgtggggttcccacttacgctccatctgcccgagtggtgaatggtgaaagcgccaaaccctacagttggccttggcaggtctctct
gcaggtcctgaaagatggggtatttttacacaattgtgggggaaccctcatcgccgacagatggatcctgactgccgcccactgtatcaacttctcccgg
accaatcgggtcgtggttggagagtttgacttagccaatgaggaaggagcagaggagatcttccttattccatctgaggacatgtttgtgcaccaatcat
ggaacagtgtttgtgtcgcttgtgggaatgacattgcccttatccgattgccccgccctgtgcagataagtgataaggttcaactgagctgcctcccccc
cgccggggaactgctccccaacaatttctcctgctatgccagtggctgggggcgactttacactggggggcccattcctgatatcctgcagcaggcgctg
ctcccagtggtggattatgatcactgcagccagagagactggtggggcgccacagtgaaacgcagcatggtgtgcgccggaggggacattcgctctgtct
gcaatggtgactctgggggacccctcaactgccagggggccgatggccggtggtacgtacatggggtggccagctttgtgtctggctttgggtgcaacac
cctcaagaagcccagtgtgttcactcgcgtgtcggcctttaactcttggatccagcanaccatcagtgagaattccgtaagtggattccagtagatccat
tcc
3' EST: CX984237 [+]ggaatggatctactggaatccacttacggaattctcactgatggtttgctggatccaagagttaaaggccgacacgcgagtgaacacactgggcttcttg
agggtgttgcacccaaagccagacacaaagctggccaccccatgtacgtaccaccggccatcggccccctggcagttgaggggtcccccagagtcaccat
tgcagacagagcgaatgtcccctccggcgcacaccatgctgcgtttcactgtggcgccccaccagtctctctggctgcagtgatcataatccaccactgg
gagcagcgcctgctgcaggatatcaggaatgggccccccagtgtaaagtcgcccccagccactggcatagcaggagaaattgttggggagcagttccccg
gcgggggggaggcagctcagttgaaccttatcacttatctgcacagggcggggcaatcggataagggcaatgtcattcccacaagcgacacaaacactgt
tccatgattggtgcacaaacatgtcctcagatggaataaggaagatctcctctgctccttcctcattggctaagtcaaactctccaaccacgacccgatt
ggtccgggagaagttgatacagtgggcggcagtcaggatccatctgtcggcgatgagggttcccccacaattgtgtaaaaataccccatctttcaggacc
tgcagagagacctgccaaggccaactgtagggtttggcgctttcaccattcaccactcgggcagatggagcgtaagtgggaaccccacatccataggctc
ctg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: