XB-CLONE-3537315
Clone Name: IMAGE:7720481
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Gene:
adsl
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX984240 [+]
tgactcccgggcccgaagaggttatgaggtataggtcgccgctggtctccaggtacgccagccgggaaatggccttcaatttcagtgacagcaagaaatt
tcagacgtggagacggctttggttatggctggggcaggcccaacggagtttggggctacccattacagaagaacagatccaggagatggaggccaacctg
gaaaatattgactttaagatggcagcggaggaagaaaagcgcttgcggcatgatgttatggcacatgtacacacgtttgcacactgctgccccaaagctg
ccccagtcattcatctcggagccacttcttgctacgtgggagacaacactgacctgattgttctacgtgatggttttgacctgctgcttccaaagcttgc
tcgagttataagccgcttggctgattttgcagaaagatacgcagagatgccaacgttaggattcacccactaccagccagctcagctcacaactgtgggg
aagcgtgcatgcctttggcttcaggacttgtgcatggacttgcgcaacttggagcgagccaggaatgaccttcgcttccgaggagtgaaaggtactacag
gcacacaggccagcttcctgcagctttttgatggtgatcatgacaaggtggaggaattggacagaatggttacttctaaggcaggatttaaaagggctta
tatagtgactggacaaacttacagccgcaaggtagatgtagaggtgctgtctgtcctggctagtttaggggcgactgttcacaagatttgcactgatatc
cgtttactgncaaacctgaaagagcttgaagagccatttgagaaagaacagatcggntcta
3' EST: CX984239 [+]tctgattgacacatttcacaggtccagctcaatcttcacatccattttactctgataaggactcagcagtggaatcacctcctcctttaaaaacttcagc
acctgctgaggtgcccggcctgtgaaggacttcggatcaagcagctgttccagatgagcatgaattggggcaaagtaagaatcagattggattctgaaga
taaggtcattgtctcctccttcttgttttaccactgcagccgcttgctgggagagaactcggattcgctcatggcagtcctgcctgtttcctccattctt
caccattgccataatgatattttctgtggccataaagggcagctcttggcggattcttctttcaatcacttttggatacaccaccaatccttccgaaatg
ttctgcagagtgctaagaatgatatctgcagtgagaaaggcttctgccaaacatactcgtctgttggcactgtcatctagtgtgcgttcaaaccactgaa
ctgaggcagtctgtagcgggttcatgatgagagtcataaggtggcgagccaagctgcagcagcgttctgagcgcatggggtttctcttgtagggcatggc
actagaaccgatctgttctttctcaaatggctcttcaagctctttcaggtttgccagtaaacggatatcagtgcaaatcttgtgaacagtcgcccctaaa
ctagccaggacagacagcacctctacatctaccttgcggctgtaagtttgtccagtcactatataagcccttttaaatcctgccttagaagtaaccattc
tgtccaat
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: