XB-CLONE-3537286
Clone Name: IMAGE:7720579
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Gene:
XB5758585
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX984259 [+]
cttcagtggttttatttgatacattttacacacaaaatagcactggtatctattgttgtgtattgcatacaatatgtggtattttatttcatgctacaat
aaatggtgatgtatagtaagtgcctttcaggctctgagctgtttcagttgttgttaataatattctgcatccagttggtaaagttacagactttaaggta
cactcctggaatacctcttcccgagcagccatttccccacaatattattccgtagagctctcccttacaaatgagaggccctccggcatcaccctggcag
gagtccttgtcgtcttgtgcaaagccagcacacattaaatttgtatgcatttttttgggactgaagtaagcgtcacagctggattcaggcagaataggta
aatccagacactgcagtctatcagggtacttctcagcatatgaattcagatttccgaagccagacacctgacaaactttcccctctcttgggcagctgct
tgcaactttgatgggctgaacaaactgattaaattgagctggctcagcaagtttaaccaacatgatattattgctatcagaaaggcccaggtatctgtta
tgtggaaaggttttctcaacctggatatgttgttctgttccttcttccctggtaatatcatgctttccaaggtgagcgatcaagtacttgggtgactggt
tgcaactggcggcagaaatgatccacctcgatgagattaaggaacctccacaccagggatatcctttataggtgaaataaacctgccatttttgggagtg
aggggcacattcatattccccgataattttatcatcaa
3' EST: CX984258 [+]tcctttggttgatgataaaattatcggggaatatgaatgtgcccctcactcccaaaaatggcaggtttatttcacctataaaggatatccctggtgtgga
ggttccttaatctcatcgaggtggatcatttctgccgccagttgcaaccagtcacccaagtacttgatcgctcaccttggaaagcatgatattaccaggg
aagaaggaacagaacaacatatccaggttgagaaaacctttccacataacagatacctgggcctttctgatagcaataatatcatgttggttaaacttgc
tgagccagctcaatttaatcagtttgttcagcccatcaaagttgcaagcagctgcccaagagaggggaaagtttgtcaggtgtctggcttcggaaatctg
aattcatatgctgagaagtaccctgatagactgcagtgtctggatttacctattctgcctgaatccagctgtgacgcttacttcagtcccaaaaaaatgc
atacaaatttaatgtgtgctggctttgcacaagacgacaaggactcctgccagggtgatgccggagggcctctcatttgtaagggagagctctacggaat
aatattgtggggaaatggctgctcgggaagaggtattccaggagtgtaccttaaagtctgtaactttaccaactggatgcagaatattattaacaacaac
tgaaacagctcagagcctgaaaggcacttactatacatcaccatttattgtagcatgaaataaaataccacatattgtatgcaatacacaacaatagata
ccagtgctattttgtgtgtaaaatgtatcanataaaaccact
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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