XB-CLONE-3537205
Clone Name: IMAGE:7720540
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Gene:
ezr
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX994897 [+]
attactacaaggtgaaaggagtagacgcataactgaaaatgccaaaaccggtaaatgttcgtgtgaccactatggatgcggagctggaattcgccatcca
accaagcaccacagggaagcagctttttgatcaggtgattaaaactgtgggcctacgtgaggtttggtactttggactgcagtgcatagacaacaaagga
tacctaacttggctgaaacttgataaaaaggtctcagcacaagaagtcaagaaggaaaaccctctgcagtttaaattccgtgccaagttttatcctgaag
atgtttctgaggaactcatccaggaaattacccagaaactattttttctgcaagtaaaagaagctattctgagtgatgaggtatactgtcctccagaaac
agctgtccttctagcttcttatgctgttcaggccaagtttggagactacagcaaagaaactatcaaatctggttacttgacctcagagcgacttcttcct
cagagggtattggatcaacacaacctttccagagaccagtgggaagaaaggattgaggtgtggcactcggagcaccgtggtatgctgaaagaggatgcta
tgctggaatatcttaagattgctcaagatctggagatgtatggtatcaattactttgaaattaaaaataagaaggggagtgacctctggctgggagtaga
tgcccttggtttaaatatttatgagcacaatgacaaactgacaccaaagattggtttcccctggagtgaaataaggaatatttctttcatgancagaagt
ttgttatcaaacccattgacaagaaag
3' EST: CX994896 [+]caaaattgtgcactgctgatatatagtataggtattctcttacaggcaggttgcctgtattaattctctccaaatgacaatcttacatattaaaaattta
taattaataaagtaattaaaaatcccttcattcttggagtctaatagcaattctttcaaataagtaaggtataattacatgtactgattatgcattaaag
gaaatcttattttaccaattactcttttagcaaactggtctactgtaggtaattcattaatttactttatatagtattatttgtaaaaataacaaaaaat
atacaacgcaaactattctttctttaatatgttacaatggatatatctcactataatatttatacataaatactataatctatgcctgattagataatac
atatgaattttacataaattgtaactttagaacatcccatggaattccccaaaagttaagtgtgggtagagactgactaattttacagttcaaagatgga
tcccaaaatgcatgatgattgcttttttttgtttcattttgaccaatggaatgttacttgcataagagagtgtgatatatactagtgttggatgaaagtt
aattgcagcctacatatcttttaatggatcattttccctggctccatagaccactagtgaataaagtaaatgaagtttgctagacaagtataacaattag
ctgcaacagggtagaaaagaattatacaaatccaacaccaatatttcacttttatcagcctttattgacacatttgtgtgatttttatcacgaatggtgt
ggcttaatggtatacttgtctttacaagcaaaaaactgcaatttcctgccaaaagtaagaccacctaaaggacaatttgcaatttctagatcattttgta
agctttgtaaataatgtccaagcgtcattaattgccagtaaacta
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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