XB-CLONE-3537097
Clone Name: IMAGE:7720502
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Gene:
dapl1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX994969 [+]
atctggcccaaatgaggatgatcctattgtttggcccctggaccaacaacaagatcttaatggaagagccggtaaatgtaccaatgtggtctgatgggat
ttttagatcagcccagcagatatctggctaatttagggtaagatgttggtaaggaaggctatcgggaaggctccatatacagccattaagcagtctggag
tggtcaagtcaccagtctttgtattgggccaatgcagaaccccagactgacagacacaggcgtttcacataaataccctgagaggagacgcttgggattt
tggctcacaccctgcagattgaagactctaagctgaataagaggctacagttggctgtgcttctaattacccatctgtcactaacatttcatcactttgc
tgcgtcccattcacatgcagtcggagaagggaatacagtctggcacctcgctttgtcagcgctccgggtgggattcttacggcatgtaactgagtggcac
ccgaacagacacttgtctaaccaaccatgattataaaaccttgataacgtgtactttgtcactcctctcccaataatggacacaattacttttaaagggt
aactatctcggtaaaaaccaagactttataaataatggggaaagtttgattacatagtgtttcattagcttgttagatattgggtactttaaattggctg
ttttcacacaatgaaaatgaatccccctccttattgtatgatattgngagaataatgttaccctattagggcacattagtgatatgaccatacaaatttc
ctcaggaagctc
3' EST: CX994968 [+]taatgcaaagtgctttatcaaaactggtacaaaacaatgattattctgggaaaagtgacttttttaacttatgtacatgacaccagagtacatgcagtct
aacatctgcaaggctgctgaataatgtacagcctttttggcagaatgatcttctcgacagtaggtcgtggttttttgtgcgcaatctgcgcggcttctcc
gggaaagtcatggctgagctgcagcgtagaaagaacagtaataagcagaagtcttcatcctgactttaccaaaaactcattactaagatacaggattttt
catagattgcctaatgccagaacaaaatattgtatcgccaaatggccacagcagtagtctatagagctgaatgaataaatatggccttaaagatctgctg
gaacccttatctagtttaacaatgtcataacagccgtcattactttcaccaaatagtgaagcataaaatatatgatataatagtttaaattatatatata
tatatatatatatatatgctatttttaatagggaggccagaacacttgaaaattcaggttcatgcagatccagctgccagggttgaactgcagtttaatt
taaagtcaatcagtgctgccctgcaacatgtatttattagacatggccctgatattttcaaggaaacggtaatgaatgaatgccaaaatagacacagcca
gtacataccttctctagctctccagccaaaatgttcattgcttgcatcttcgtcatgttaagaacagagctaanagaatagaatanaaatcagtgtacta
aagattacagactgccttgtatttccactt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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