XB-CLONE-3537085
Clone Name: IMAGE:7720312
|
Gene:
ckb
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX994977 [+]
caagactaagtctagtgcaatcatgcctttcggtaactctcacaacaccttgaagctgaagaactctgtggatgaggagttcccagacttgtctgcccac
aacaatcatatggccaaggtgctgacaaaggatctctacgcgaaactgagggataaacagacacctagtggatttaccgtggatgatgtcattcaaactg
gggttgacaacccaggccatccctttattatgacagtgggatgtgtcgctggggatgaagaatcttatgaagtctttaaggagctctttgaccctatcat
tgaggacagacatggtggctacaagccaacagatcagcacaagactgacataaattcagaaaacctaaagggaggtgatgatctggacccaaactatgta
ctcagctctcgggtcagaactggaaggagtattcgtggattcagcctcccacctcactgcagccgtggagaaaggcgtgcaattgaaaagatgtcaattg
aagcacttgctagcttggatggggacctaaaaggaaaatactacgctcttaataccatgactgatcaggagcagcagcagcttatcgatgaccacttcct
gtttgataagccagtttctccactcctcttggcctctggaatggcccgtgactggcctgatgccagaggaatctggcacaacgacaacaagaccttcctt
gtctggatcaatgaggaagaccatcttcgtgtgatctcaatgcaaaagggtggcaacatgaaggatgtgttccagaggttttgcactggccttacaaaga
ttgaagctatcttcaagaacaaaggatctgcattcatgtggaaccagcatcttggttatgttcttacctgcccat
3' EST: CX994976 [+]gaggccagccatcaaaagaaagataattctaagaaacagatggaagacatttctacagtgttactaagcaggttgggtccccagggtcttggagtgtagc
tatttcatccaataaattagaaagcatggccaataaagtgcagttatttctgtgctggaatgaggtcatcgatggactctcctttttccaggcatttttc
catttcaacaaggagttttacaccatcaaccaccatttggaccagttcaacctcagagaaacccagacgatcagcattggagatatcaaacaccccacca
acggctgcagtgtctacaccacctgttcctctcttctgtagtctaagtctcttcaggatttcaccaaacttctcattcttgcttaagtttggcaacttaa
tgtgaacaccagccctcagaccagtaccgaggttggatgggcaggtaagaacataaccaagatgctggttccacatgaatgcagatcctttgttcttgaa
gatagcttcaatctttgtaaggccagtgcaaaacctctggaacacatccttcatgttgccacccttttgcattgagatcacacgaagatggtcttcctca
ttgatccagacaaggaaggtcttgttgtcgttgtgccagattcctctggcatcaggccagtcacgggccattccagaggccaagaggagtggagaaactg
gcttatcaaacaggaagtggtcatcgataagctgctgctgctcctgatcagtcatggtattaagagcgtagtattttccttttaggtccccatccaagct
agcaagtgcttcaattgacatcttttncattgcacgcctttctccacggctgcagtg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: