XB-CLONE-3537056
Clone Name: IMAGE:7720458
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Gene:
cdhr2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX994999 [+]
cggaattctttcattggatcctgatgtgatgcttgattacgagacacagaaaaactataccttaataattgaagctcaagataatgggcttcacggagta
agtcacacagcaactgctactgccagtgtccaagtgctggatctcaatgatgagccgccatatgttgacccctcctctctaaacaacctgtttcttcttg
agaacagaactgtgggtctagagttacttaccactctgaaggcgcatgatcctgataccatccacgaattggaatttcaagctctctcattgcagtgctt
caagaatggcaatgatataggcaacatttgctatgactggatctggttggctccaaatggggaactttttgtaaatgacactgaagttgttgactatgag
ctctgtgatgtgatggtgatgatgctgcgtgtagaggacaagctgacattgattggggacagatacagcaaaaatgaatccctgcgtgtggacatactag
atgtcaatgacaatgctccagagttcttggatgtcgatgaagtatttgtggttgtgcctgatgtagcacctattgattaccaagtggctcttgtcaaggc
caccgataaagactctggcttgaacgctgttcttaagttttcatcgcctcagttgaatttatctcaggtgacagcacaagaccacttcttaacatcttca
ctattgtcaccactagtgaaaaggaaatctaccagggcagtatcagggttgcaagctcattggatgccaccttgaagggtaattatcgagtaactgtgat
tgctgaggatt
3' EST: CX994998 [+]ataagaaaaacttaatttattttgagcaatgaataggtacagaatatacatgtaaagctgaatgtttttcatttgttttcaaccagtggctcacaagcaa
catgtttctcagcgccctctttcatgttgctcccagagatttactccaggcagatcctcctgtaggataacagtcacatcggactgccaaatagccaatc
acagcccttatttggcatctacaaagaacttatttcatgcttgtgtggctcaccaacactttttacatctgtgtgtggctcacaagtaaaaaaaggttgg
gggtttctgtactagaggataaaactaattttaaagcagaaaggtaccattttaaatatacttaccccaaataagggcaaaactctgaaactgcaataca
tatgaagtgattataatctaattgcataactctactaatccagctactgatggactacagttctgataattagatatatacaaaggtttctgggtgttgt
agttgtcataagtaggttgacattgcttaggcatgttatgtttcatgagcatttatattcacatgttcaggggacctatgagttgaaaggcatttcaatg
ttacttccatgcagttctttctgtagagagcaaacaaatagcaatggcaaacaaataaaacaaatagcaatggcacactgccaccagtgcttggattggg
gcacaacatcttgcaataaaaaaaacaattctcttttccaaaatattatcattatgtaactgtacctagggtacatagtgatcagcacacagctcatcta
aactcaaggactgnggttgcatgagtgacaagtgacccccacaataaataatggatgtcttgccatattgggc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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