XB-CLONE-3537011
Clone Name: IMAGE:7720510
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Gene:
atp1a1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX995030 [+]
accggctgcttctcttactaccaagaggcaaagagctctaagatcatggagtccttcaagaacatggttcctcagcaagccctggtaatccgaaacggag
agaagttgagtattaatgcggaagacgtggttttgggtgacctggtagaagtgaagggaggagatcggatacctgcggatgttcggatcatctccgctca
tggctgcaaggtggataactcttccctcactggagagtccgaaccccagacccgctctcctgacttcaccaatgagaacccactggagacccgcaacatc
gccttcttctctacaaactgtgtggaaggcacagcccgtggcgttgtcgtcaacaccggtgatcgtacagtgatgggccgtatcgctactctggcctctg
gcctggagggaggacgcacccccattgccattgaaattgagcattttattcacatcatcaccggcgtggcggtcttcctgggggtcagcttcttcatcct
gtcactcattctgcagtacacctggctggaggctgtcatcttcctcattggtatcattgttgccaatgtgccagagggactgctggctactgtcacggtt
tgccttacactgaccgccaaaagaatggcccgcaagaactgtcttgtgaagaatctggaggctgtggagactttgggatccacttccaccatttgctccg
acaaaacgggcaccctgacacagaaccgaatgaccgtagcccacatgtggtttgataaccagatccatgaagccgacaccacggagaatcagagcggtgc
ctccttcgataagagctcccccacatggacagccctgtcccgggttgctggattgtgcaac
3' EST: CX995029 [+]aaaaatgcagatatataaaacatctctgaacgtgctcccattacaaaaagaaacaaaaaaaagaaacaaacgaaaaacaataccggagaaggcacaatga
gaactctgtacaggacgcacacaggcgacactgcgccccttgccaaaatgcatctcttccatgggaacaggctgagtcagacgggtgcacgcaggcgacg
ggtgggtgcaggcagaccgaggggcaggaagtgacgtgaaatgttcggacgacccgaaggctctaatagtaggtttctttctccacccagccgcctgggc
tgcgccggataatcagcttacggacttcatcgtatatgaagatgagcagcgagtatgggaaggcacagaaccaccaggtcggcttgagtgggtacatgcg
caatgccacatccatgccagggcagtaggacaggaaggcagccagtgcagtctcttcaaacagcccaaagataaggattttattcttcatgccctgctgg
aacactgaattccttctggtcttgcagatgatcaaatcggcccactgcaccaccacgatactgacgaagaaagccgtatggcacgtgaactccacaatct
tcctctgctcgtaggtccactgctgcccgtaactgtcctccacgtcgttattccagcggtcatcccagttcactctgatacccagcagtgtccagggcag
aaagccattctcagccaggatcacaaagtatgtgaagaacccccccagggcttggatcataccgatctgcccataggccatactgataagcctttcattc
accagcttgtctgttttgggattacggngctgtctcttcatgatgtcactctcggcttgctcataggc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: