XB-CLONE-3536996
Clone Name: IMAGE:7720368
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Gene:
dapk1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX995040 [+]
ctgtatcgctcctaatgtctaaactgagagaacttgggcgcagggatgcagcagattttttactaaaagcatcctctgtttttaaggtgactcttgatat
ccatggacaagaagcctatgcggcaagctgcaacagcggcacatcctataattcaattagctcagtcgtgtcacgatgagtaaattagtccgcatgatct
tctatagctatctaaacaaccagtatgagaagtgtagtttatatgtgtttcataggggtttctaaccattaatttataaacaatgtcaaagaatgccaca
tgccatcagaacctggtagctttctactttttaataagggtgactgccttttatcatatattttaaaggctcccatgaatttttcactccagagttctat
aataatgtgctgctagttactgccacctgaacagataatatatactgttcattttttctaattaattcttttatacattttactaaccagactggacagt
tgttcacgtgtagaagagagggctctcattttatgtctgctgatttggtaactgattcagctacagatgatagcaaattttgctgtacattagagttgcc
caacctgcagacctcaagctcttggataatgccattttactttatcagtagctggcagagcccaggttggacagccccctctgtttatattcataaagcc
aatacagcagcatgagtgccttgctctcacaggttaggttacaataggccagaacctgctctncagcactttccactgggctctacctctctatcgttgt
ctccacgataatccttgtgtc
3' EST: CX995039 [+]tcaacaggcaacatgtttattcatatatatttacaaatataaaaagtttaaaattgtactacaaataaaacattaataaatggaaaggttactctcttta
tatacatgtacatatgtccattcataacacatgcagcttccaaagataaatgtaatttcccccttccacattggctgtgttttataaaccaaagcaactg
accttgcttctttgtttcaagtacaagaaatcaaataatgcaccctagatgctgcctgttgttgtgtgcaaactataataactaataatataaatggtcc
ttgcagtgatttaaataaacagtaatgctttacataaaatatgtctatatgaacgttcaacaccaggtttttaatagaccccttgcatttataatactca
gcgattgccagacagtcccagctaactctatcctactgcttctttggtcaataacagggaataatgccacacacaaatggctcatgaccccttcagtctt
tgcacagagatttcttttcattagaacagttaagttagtgaaaattgcttaaaaggaatgttggcaagtacagattagcaaggaaataattgtataagag
tcatggaagtttcctttttaaccaatagagtgaagaaacataatagcagctccgtgtgtcttcacccctaagctgatggcccacagagcgagccagtcac
ctgcaatgatctctactaccacaggcgatcaacttccttttcaccgacaacaataggagccgccagcggaaaggcattttggacgggttaattacctgtg
atgcagagatctatcgcaggcgaccaactcgctccacgggccatcagccttaaggtcctttttacaatacaaaactaagcatacaaatgaggataagtag
aactca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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