XB-CLONE-3536932
Clone Name: IMAGE:7720422
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Gene:
ctdspl2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX995082 [+]
tatatcatgaagtttatccagtgcaaaaaataaaatacaatttgacatccaattttatgtattatttacacattatgattaaatatctatttctttatta
ccctgtaccaaaatatacaaatgtacaagaatcaacaaaatatatgtttagccaggtgtaaacactgtacaagtctttcaaaacaaaaaaatatatactt
cacagccacaaatctccctctcatctatagcactgccctaccaggtcaaacccagcttgcccaattatgcagccttatggttgaaggctcacagcaggcg
tatacccatgtgttattacatgttccattttctgttgtgtgtgcaagctgcttggaacctaaacacagtagcagtagcagtacctcaggttctccacact
aacctgactgctacatactatttcaaaaattgtggctttacctggggatcaccataatcacttttagcccttaggtgggttctattcctcctaacttctc
acttgctagcctattactactgtactatgtagggggtaatacataggccaaagaggcgattcctctccaccatgctccttatataagctctaagcctttg
gtcttcccattttctaagaagtttgtgggaggcctagcaaagacccaacctagggtgtagctactgcctaaagtctaaggttgaaagtacaattaaaatg
gattatattaagggctatggcacacctggacattagtcaaccataataaatatcctctactgtgggcggcttatctcctgcaaatgatttcccactggca
atcatgtaaatcgccacaaggcaactttgggctaatttggcaaatcaaagcgatttataccaaaaccaaacctgt
3' EST: CX995081 [+]ggaaatcagttgcctgagattcagttcacagagctacaaaacgccaccattcacaacactacccactggacctcaagcattacattctctggcaatcaca
ccgtcgagccgactggatgtcctgtagtacagacctggtgcttgtatactcctattatccatctgggccagtacctcacctctgacattcttattggtgt
aggatacccaatctgtaatgtaatgtcatacaccctatactccaagatcataggcccaaaaccacagggattgtatatgggctggttaacagcagctggc
agtgcagcccgcaccttgggaccagtattcgtcagccagatctacacacatctgggaaccagatggacatttggtatcatttgtgtaattgttgccttgt
ctttgctacacttaactgctgtatataaaagacttacccctttttctgctcgatatgaaaggctatagcttggatacacattgaactacatgttacacta
tttgctcggtaattcatcatacagaacagtgttaaacaaaacaacacaatttcaggtagccctttttaacattttaaaggagaaagaaaggtaaaaacta
agtaagctttatcagaaaggtctatgtaaatacagccataagcactcacagaaacgctgcactgacttctctgaaaaaagattagttgtgtctgtaattc
ctctaccagagacatgcacctttctgttctctgctctttcctgcttccccctccctncagaatgctaagaactcacccccctccccttaggaatgtggat
ctgagccaatcc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
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